UHPLC-ESI-ORBITRAP-MS analysis of the native mapuche medicinal plant palo negro (leptocarpha rivularis DC. – asteraceae) and evaluation of its antioxidant and cholinesterase inhibitory properties
Author
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Jiménez-González, Andrea
Author
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Quispe, Cristina
Author
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Bórquez, Jorge
Author
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Sepúlveda, Beatriz
Author
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Riveros, Felipe
Author
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Areche, Carlos
Author
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Nagles, Edgar
Author
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García Beltrán, Olimpo
Author
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Simirgiotis, Mario J.
Admission date
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2018-10-11T15:07:02Z
Available date
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2018-10-11T15:07:02Z
Publication date
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2018
Cita de ítem
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Journal of Enzyme Inhibition and Medicinal Chemistry 2018, VOL. 33, NO. 1, 936–944
es_ES
Identifier
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10.1080/14756366.2018.1466880
Identifier
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https://repositorio.uchile.cl/handle/2250/152088
Abstract
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UHPLC/ESI/MS identification of organic compounds is the first step in the majority of screening techniques for the characterization of biologically active metabolites in natural sources. This paper describes a method for the fast identification and characterisation of secondary metabolites in Leptocarpha rivularis DC. (Palo negro) extracts by HPLC/UV (DAD)-Mass Spectrometry (HPLC/MS). The plant is used for the treatment of several diseases since pre-hispanic Mapuche times. Thirty-seven compounds were detected in the aqueous edible extract for the first time including 4 sesquiterpenes, 10 flavonoids, 9 oxylipins, 2 organic acids, and 11 phenolic acids. In addition, phenolic content antioxidant and cholinesterase inhibitory activities were measured for the first time using the edible infusion. The total polyphenol content of the infusion was 230.76 +/- 2.5mmol GAE/kg dry weight, while the antioxidant activity was 176.51 +/- 28.84; 195.28 +/- 4.83; and 223.92 +/- 2.95mmol TE/kg dry weight, for the DPPH, ABTS, and FRAP assays, respectively. The cholinesterase inhibitory activity was 7.38 +/- 0.03 and 5.74 +/- 0.06mmol GALAE/kg, for the inhibition of acetylcholinesterase AChE and BChE, respectively, showing that this plant is a candidate for the isolation of compounds that can be useful for the treatment of neurodegenerative diseases. Furthermore, this plant could serve also as a raw material for the production of dietary supplements, due to its content of polyphenolic compounds.
es_ES
Patrocinador
dc.description.sponsorship
FONDECYT
1180059
FONDEF
ID17AM0043
INACH
RT 16-17
Universidad de Ibague-Gobernacion del Tolima
17-477-SEM
16-443-SEM
Journal of Enzyme Inhibition and Medicinal Chemistry
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Keywords
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Electrospray
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Keywords
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Antioxidants
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Keywords
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Metabolomics
es_ES
Keywords
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Orbitrap
es_ES
Keywords
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Leptocarpha rivularis
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Keywords
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PueblosOriginarios_Uchile
Título
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UHPLC-ESI-ORBITRAP-MS analysis of the native mapuche medicinal plant palo negro (leptocarpha rivularis DC. – asteraceae) and evaluation of its antioxidant and cholinesterase inhibitory properties