Attenuation of high-fat diet-induced rat liver oxidative stress and steatosis by combined hydroxytyrosol- (HT-) eicosapentaenoic acid supplementation mainly relies on HT
Author
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Echeverría, Francisca
Author
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Valenzuela, Rodrigo
Author
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Bustamante, Andrés
Author
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Álvarez, Daniela
Author
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Ortiz, Macarena
Author
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Soto Alarcón, Sandra
Author
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Muñóz, Patricio
Author
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Corbari, Alicia
Author
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Videla Cabrera, Luis
Admission date
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2018-11-30T12:38:44Z
Available date
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2018-11-30T12:38:44Z
Publication date
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2018
Cita de ítem
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Oxidative Medicine and Cellular Longevity Vol. 2018, Art. ID 5109503, 13 pp.
es_ES
Identifier
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1942-0900
Identifier
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10.1155/2018/5109503
Identifier
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https://repositorio.uchile.cl/handle/2250/153018
Abstract
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Pharmacological therapy for nonalcoholic fatty liver disease (NAFLD) is not approved at the present time. For this purpose, the effect of combined eicosapentaenoic acid (EPA; 50 mg/kg/day) modulating hepatic lipid metabolism and hydroxytyrosol (HT; 5 mg/kg/day) exerting antioxidant actions was evaluated on hepatic steatosis and oxidative stress induced by a high-fat diet (HFD; 60% fat, 20% protein, and 20% carbohydrates) compared to a control diet (CD; 10% fat, 20% protein, and 70% carbohydrates) in mice fed for 12 weeks. HFD-induced liver steatosis (i) was reduced by 32% by EPA, without changes in oxidative stress-related parameters and mild recovery of Nrf2 functioning affording antioxidation and (ii) was decreased by 42% by HT, concomitantly with total regain of the glutathione status diminished by HFD, 42% to 59% recovery of lipid peroxidation and protein oxidation enhanced by HFD, and regain of Nrf2 functioning, whereas (iii) combined EPA + HT supplementation elicited 74% reduction in liver steatosis, with total recovery of the antioxidant potential in a similar manner than HT. It is concluded that combined HT + EPA drastically decreases NAFLD development, an effect that shows additivity in HT and EPA effects that mainly relies on HT, strengthening the impact of oxidative stress as a central mechanism underlying liver steatosis in obesity.
es_ES
Patrocinador
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Entidad financiadora: Fondo Nacional de Desarrollo Cientifico y Tecnologico (National Fund for Scientific and Technological Development) - 11140174
Attenuation of high-fat diet-induced rat liver oxidative stress and steatosis by combined hydroxytyrosol- (HT-) eicosapentaenoic acid supplementation mainly relies on HT