About
Contact
Help
Sending publications
How to publish
Advanced Search
View Item 
  •   Home
  • Facultad de Ciencias Veterinarias y Pecuarias
  • Artículos de revistas
  • View Item
  •   Home
  • Facultad de Ciencias Veterinarias y Pecuarias
  • Artículos de revistas
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Browse byCommunities and CollectionsDateAuthorsTitlesSubjectsThis CollectionDateAuthorsTitlesSubjects

My Account

Login to my accountRegister
Biblioteca Digital - Universidad de Chile
Revistas Chilenas
Repositorios Latinoamericanos
Tesis LatinoAmericanas
Tesis chilenas
Related linksRegistry of Open Access RepositoriesOpenDOARGoogle scholarCOREBASE
My Account
Login to my accountRegister

A detection method for infectious pancreatic necrosis virus (IPNV) based on reverse transcription (RT)‐polymerase chain reaction (PCR)

Artículo
Thumbnail
Open/Download
Iconitem_0028156345.pdf (2.011Kb)
Access note
Acceso a solo metadatos
Publication date
1994
Metadata
Show full item record
Cómo citar
López Lastra, Marcelo
Cómo citar
A detection method for infectious pancreatic necrosis virus (IPNV) based on reverse transcription (RT)‐polymerase chain reaction (PCR)
.
Copiar
Cerrar

Author
  • López Lastra, Marcelo;
  • González Canales, Marcelo;
  • Jashes, M.;
  • Sandino García, Ana María;
Abstract
A rapid, sensitive and highly specific detection method for infectious pancreatic neerosis virus (IPNV), based on reverse transcription (RT) polymerase chain reaction (PCR) has been developed. The specificity of the assay is provided by the oligonucleotide primers selected from the IPNV major capsid polypeptide VP2 gene. For each primer combination only one major product is obtained when amplifieation is performed using IPNV double‐stranded RNA from two different viral strains, Sp and VR‐299, as the initial template. No products were detected when genomie nueleic acids other than IPNV RNA were used as RT‐PCR templates. The specificity of the amplification products were confirmed by Southern hybridization using a specific cDNA probe. To assess the sensitivity of the method, dilutions of purified IPNV dsRNA total genome were amplified and quantities of as little as 1 pg of purified dsRNA were detected when the amplification product was visualized by silver‐stained polyacrylamidc gel electrophoresis. This technique detected IPNV directly in infected coho salmon, Oncorhynchus kisutch (Walbaum), and rainbow trout, Oncorhynchus mykiss (Walbaum), tissues and fish egg samples, avoiding viral propagation in cell culture. The results show that this RT‐PCR amplification method is useful for the direct tissue detection of IPNV
Indexation
Artículo de publicación SCOPUS
Identifier
URI: https://repositorio.uchile.cl/handle/2250/157205
DOI: 10.1111/j.1365-2761.1994.tb00222.x
ISSN: 13652761
01407775
Quote Item
Journal of Fish Diseases, Volumen 17, Issue 3, 1994, Pages 269-282
Collections
  • Artículos de revistas
xmlui.footer.title
31 participating institutions
More than 73,000 publications
More than 110,000 topics
More than 75,000 authors
Published in the repository
  • How to publish
  • Definitions
  • Copyright
  • Frequent questions
Documents
  • Dating Guide
  • Thesis authorization
  • Document authorization
  • How to prepare a thesis (PDF)
Services
  • Digital library
  • Chilean academic journals portal
  • Latin American Repository Network
  • Latin American theses
  • Chilean theses
Dirección de Servicios de Información y Bibliotecas (SISIB)
Universidad de Chile

© 2020 DSpace
  • Access my account