Effects of fat source and copper on unsaturation of blood and milk triacylglycerol fatty acids in Holstein and Jersey cows

Abstract

Fatty acid composition of plasma triacylglycerides and milk fat was analyzed from Holstein and Jersey cows with control or depleted copper status and fed roasted whole soybeans or tallow. Conjugated linoleic acid in plasma was higher in Jersey cows. Dietary fat source influenced the proportions of all fatty acids in plasma and in milk, except for conjugated linoleic acid in milk. Feeding soybeans increased plasma C14:1, C18:0, C18:2, and conjugated linoleic acid, and decreased C14:0, C16:0, C16:1, and cis-and trans-C18:1 compared with feeding tallow. Low copper diets decreased C18:0 and increased cis-and trans-C18:1, and conjugated linoleic acid in plasma. A fat source × copper status interaction occurred for cis-C18:1 in plasma. Proportions of C4:0 to C14:0 were higher, and cis16:1, cis-and trans-C18:1, and conjugated linoleic acid were lower in milk fat of Jersey compared with Holstein cows. Generally, the effects of copper depletion were less apparent in milk than in plasma. Copper depletion increased C4:0, trans-C18:1, and conjugated linoleic acid, and decreased C16:1 in milk. Feeding whole soybeans increased C4:0 to C14:0, C18:0, C18:2, and C18:3, and decreased C14:1, C16:0, C16:1, and cis-and trans-C18:1 in milk. Breed × fat interactions occurred for C4:0, C14:1, C16:1, and conjugated linoleic acid in milk. Copper status × fat source interaction occurred for trans-C18:1. The breed × copper status interaction was apparent in milk fat for C16:1 and C18:0 and conjugated linoleic acid in milk. Both C18:0 and trans-C18:1 were desaturated by mammary tissue; however, whereas desaturation of C18:0 was linear, desaturation of trans-C18:1 reached a plateau that could have been caused by presence of the trans-10 isomer, which is not desaturated and was not separated from trans-11 C18:1 in our analysis. Comparison of the plasma triacylglycerol fatty acid profile with the milk fat profile was useful to interpret separate events of biohydrogenation in the rumen and desaturation by the mammary gland