Gene expression and chromosomal location for susceptibility to Sjögren's syndrome
Author
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Pérez, Paola
Author
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Anaya, Juan Manuel
Author
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Aguilera, Sergio
Author
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Urzúa Tobar, Ulises
Author
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Munroe, David
Author
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Molina, Claudio
Author
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Hermoso Ramello, Marcela
Author
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Cherry, James Michael
Author
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Alliende, Cecilia
Author
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Olea, Nancy
Author
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Ruiz-Narváez, Edward
Author
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González Burgos, María Julieta
Admission date
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2018-12-20T15:10:08Z
Available date
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2018-12-20T15:10:08Z
Publication date
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2009
Cita de ítem
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Journal of Autoimmunity, Volumen 33, Issue 2, 2018, Pages 99-108
Identifier
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08968411
Identifier
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10959157
Identifier
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10.1016/j.jaut.2009.05.001
Identifier
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https://repositorio.uchile.cl/handle/2250/158156
Abstract
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Primary Sjögren's syndrome (SS) is a chronic inflammatory autoimmune disease affecting mainly the exocrine glands. Its physio-pathology is poorly understood and most of the knowledge has been related to the inflammatory component. The aim of this work was to evaluate gene expression profiling in fractions enriched in epithelial cells from labial salivary glands (LSGs) of patients with primary SS and identify chromosomal regions harboring susceptibility genes expressed in epithelial cells. A combined approach of gene expression and genome-wide association study was used. Enriched epithelial cell fractions were obtained from LSGs of patients and controls. Amplified total RNA was labeled and hybridized to 10K cDNA microarrays. Results were normalized and subjected to statistical and functional analysis. A genome-wide microsatellite screen at 10 cM resolution (393 markers) was performed. In salivary gland-epithelial cells from patients 528 genes were expressed differentially in comparison