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Authordc.contributor.authorMarambio, Paola 
Authordc.contributor.authorToro, Barbra 
Authordc.contributor.authorSanhueza, Carlos 
Authordc.contributor.authorTroncoso, Rodrigo 
Authordc.contributor.authorParra, Valentina 
Authordc.contributor.authorVerdejo, Hugo 
Authordc.contributor.authorGarcía Nannig, Lorena 
Authordc.contributor.authorQuiroga, Clara 
Authordc.contributor.authorMunafo, Daniela 
Authordc.contributor.authorDíaz Elizondo, Jessica 
Authordc.contributor.authorBravo Sagua, Roberto 
Authordc.contributor.authorGonzález Burgos, María Julieta 
Authordc.contributor.authorDíaz Araya, Guillermo 
Authordc.contributor.authorPedrozo Cibils, Zully 
Authordc.contributor.authorChiong Lay, Mario 
Cita de ítemdc.identifier.citationBiochimica et Biophysica Acta - Molecular Basis of Disease, Volumen 1802, Issue 6, 2018, Pages 509-518
Abstractdc.description.abstractAggresomes are dynamic structures formed when the ubiquitin-proteasome system is overwhelmed with aggregation-prone proteins. In this process, small protein aggregates are actively transported towards the microtubule-organizing center. A functional role for autophagy in the clearance of aggresomes has also been proposed. In the present work we investigated the molecular mechanisms involved on aggresome formation in cultured rat cardiac myocytes exposed to glucose deprivation. Confocal microscopy showed that small aggregates of polyubiquitinated proteins were formed in cells exposed to glucose deprivation for 6. h. However, at longer times (18. h), aggregates formed large perinuclear inclusions (aggresomes) which colocalized with γ-tubulin (a microtubule-organizing center marker) and Hsp70. The microtubule disrupting agent vinblastine prevented the formation of these inclusions. Both small aggregates and aggresomes colocalized with autophagy markers such as GFP-LC3 and Rab24. Glucose de
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.uri
Sourcedc.sourceBiochimica et Biophysica Acta - Molecular Basis of Disease
Keywordsdc.subjectCardiac myocytes
Keywordsdc.subjectReactive oxygen species
Títulodc.titleGlucose deprivation causes oxidative stress and stimulates aggresome formation and autophagy in cultured cardiac myocytes
Document typedc.typeArtículo de revista
Indexationuchile.indexArtículo de publicación SCOPUS

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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile