Ethylendiamine-functionalized multi-walled carbon nanotubes prevent cationic dispersant use in the electrochemical detection of dsDNA
Author
dc.contributor.author
Cañete Rosales, Paulina
Author
dc.contributor.author
Álvarez Lueje, Alejandro
Author
dc.contributor.author
Bollo Dragnic, Soledad
Admission date
dc.date.accessioned
2018-12-20T15:10:54Z
Available date
dc.date.available
2018-12-20T15:10:54Z
Publication date
dc.date.issued
2014
Cita de ítem
dc.identifier.citation
Sensors and Actuators, B: Chemical, Volumen 19, 2014, 688-694.
Identifier
dc.identifier.issn
09254005
Identifier
dc.identifier.other
10.1016/j.snb.2013.10.056
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/158301
Abstract
dc.description.abstract
In this paper, for the first time, an electro-analytical method based on the direct adsorption of double-stranded deoxyribonucleic acid (dsDNA) at glassy carbon electrode modified with ethylendiamine (eda)functionalized multi-walled carbon nanotubes (eda-CNT) is reported. The carbon nanotubes were func-tionalized with ethylendiamine via chemical modification of the carboxyl groups, and the eda-CNTs werecharacterized by different techniques, including Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), elemental analysis and cyclic voltammetry, and compared with pristine (CNT)and oxidized (ox-CNT) MWCNTs.The presence of eda on the CNT surface allows them to disperse in a phosphate buffer solution at pH7.0 and provides an environment that promotes the electrostatic adsorption of dsDNA. At eda-CNTs, theadsorption of dsDNA is improved and a linear correlation between the oxidation current of guanine basesand the accumulation time is observed. This result indicates that the presence of positive charges on thesurface of the nanotube plays an important role in the attraction of the dsDNA molecule.A more sensitive detection of DNA was obtained compared with CNT and ox-CNT when eda-CNTwhere used with a linear range from 5 to 60 ppm, a sensitivity of 0.0315 ± 0.0003 A mg–1L and a LODof 0.971 ppm after a 10-min accumulation which is lower than that obtained previously using cationicdispersing agents. The analytical performance reported is comparable with that reported previously forcationic polymers in terms of linear range and LOD values.