Show simple item record
Author | dc.contributor.author | Primo, Emiliano N. | |
Author | dc.contributor.author | Kogan Bocian, Marcelo | |
Author | dc.contributor.author | Verdejo, Hugo E. | |
Author | dc.contributor.author | Bollo Dragnic, Soledad | |
Author | dc.contributor.author | Rubianes, María D. | |
Author | dc.contributor.author | Rivas, Gustavo A. | |
Admission date | dc.date.accessioned | 2018-12-20T15:11:42Z | |
Available date | dc.date.available | 2018-12-20T15:11:42Z | |
Publication date | dc.date.issued | 2018 | |
Cita de ítem | dc.identifier.citation | ACS Applied Materials and Interfaces, Volumen 10, Issue 28, 2018, Pages 23501-23508 | |
Identifier | dc.identifier.issn | 19448252 | |
Identifier | dc.identifier.issn | 19448244 | |
Identifier | dc.identifier.other | 10.1021/acsami.8b03039 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/158427 | |
Abstract | dc.description.abstract | © 2018 American Chemical Society. We report the first optical biosensor for the novel and important cardiac biomarker, galectin-3 (Gal3), using the anti-Gal3 antibody as a biorecognition element and surface plasmon resonance (SPR) for transducing the bioaffinity event. The immunosensing platform was built at a thiolated Au surface modified by self-assembling four bilayers of poly(diallyldimethylammonium chloride) and graphene oxide (GO), followed by the covalent attachment of 3-aminephenylboronic acid (3ABA). The importance of GO, both as the anchoring point of the antibody and as a field enhancer for improving the biosensor sensitivity, was critically discussed. The advantages of using 3ABA to orientate the anti-Gal3 antibody through the selective link to the Fc region were also demonstrated. The new platform represents an interesting alternative for the label-free biosensing of Gal3 in the whole range of clinically relevant concentrations (linear range between 10.0 and 50.0 ng mL-1, | |
Lenguage | dc.language.iso | en | |
Publisher | dc.publisher | American Chemical Society | |
Type of license | dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
Link to License | dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
Source | dc.source | ACS Applied Materials and Interfaces | |
Keywords | dc.subject | cardiac biomarker | |
Keywords | dc.subject | galectin3 | |
Keywords | dc.subject | graphene oxide | |
Keywords | dc.subject | immunosensor | |
Keywords | dc.subject | layer-by-layer self-assembly | |
Keywords | dc.subject | SPR | |
Título | dc.title | Label-Free Graphene Oxide-Based Surface Plasmon Resonance Immunosensor for the Quantification of Galectin-3, a Novel Cardiac Biomarker | |
Document type | dc.type | Artículo de revista | |
Cataloguer | uchile.catalogador | SCOPUS | |
Indexation | uchile.index | Artículo de publicación SCOPUS | |
uchile.cosecha | uchile.cosecha | SI | |
Files in this item
- Name:
- item_85050288710.pdf
- Size:
- 2.141Kb
- Format:
- PDF
This item appears in the following Collection(s)
Show simple item record
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile