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Author | dc.contributor.author | Paredes, Aaron J. | |
Author | dc.contributor.author | Naranjo-Palma, Tatiana | |
Author | dc.contributor.author | Alfaro-Valdés, Hilda M. | |
Author | dc.contributor.author | Barriga, Andrés | |
Author | dc.contributor.author | Babul Cattán, Jorge | |
Author | dc.contributor.author | Wilson, Christian A.M. | |
Admission date | dc.date.accessioned | 2018-12-20T15:13:17Z | |
Available date | dc.date.available | 2018-12-20T15:13:17Z | |
Publication date | dc.date.issued | 2017 | |
Cita de ítem | dc.identifier.citation | Analytical Biochemistry, Volumen 517, | |
Identifier | dc.identifier.issn | 10960309 | |
Identifier | dc.identifier.issn | 00032697 | |
Identifier | dc.identifier.other | 10.1016/j.ab.2016.11.004 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/158570 | |
Abstract | dc.description.abstract | © 2016 Elsevier Inc.DNA staining in gels has historically been carried out using silver staining and fluorescent dyes like ethidium bromide and SYBR Green I (SGI). Using fluorescent dyes allows recovery of the analyte, but requires instruments such as a transilluminator or fluorimeter to visualize the DNA. Here we described a new and simple method that allows DNA visualization to the naked eye by generating a colored precipitate. It works by soaking the acrylamide or agarose DNA gel in SGI and nitro blue tetrazolium (NBT) solution that, when exposed to sunlight, produces a purple insoluble formazan precipitate that remains in the gel after exposure to light. A calibration curve made with a DNA standard established a detection limit of approximately 180 pg/band at 500 bp. Selectivity of this assay was determined using different biomolecules, demonstrating a high selectivity for DNA. Integrity and functionality of the DNA recovered from gels was determined by enzymatic cutting with a res | |
Lenguage | dc.language.iso | en | |
Publisher | dc.publisher | Academic Press Inc. | |
Type of license | dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
Link to License | dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
Source | dc.source | Analytical Biochemistry | |
Keywords | dc.subject | Blue light | |
Keywords | dc.subject | DNA integrity | |
Keywords | dc.subject | DNA staining | |
Keywords | dc.subject | Formazan | |
Keywords | dc.subject | Nitro blue tetrazolium | |
Keywords | dc.subject | Sybr Green | |
Título | dc.title | New visible and selective DNA staining method in gels with tetrazolium salts | |
Document type | dc.type | Artículo de revista | |
Cataloguer | uchile.catalogador | SCOPUS | |
Indexation | uchile.index | Artículo de publicación SCOPUS | |
uchile.cosecha | uchile.cosecha | SI | |
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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile