Show simple item record

Authordc.contributor.authorPalomino Mackenney, Jaime 
Authordc.contributor.authorReyes Solovera, Mónica de los 
Admission datedc.date.accessioned2018-12-20T15:13:17Z
Available datedc.date.available2018-12-20T15:13:17Z
Publication datedc.date.issued2016
Cita de ítemdc.identifier.citationTheriogenology, Volumen 86, Issue 6, 2018, Pages 1541-1549
Identifierdc.identifier.issn0093691X
Identifierdc.identifier.other10.1016/j.theriogenology.2016.05.013
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/158573
Abstractdc.description.abstract© 2016 Elsevier Inc. This study aimed to investigate the expression profiles of growth differentiation factor 9 (GDF-9) and bone morphogenetic protein 15 (BMP-15) mRNA in canine oocytes and follicular cells throughout development at the different phases of the estrus cycle. Ovarian structures (follicles and CL) and plasma progesterone concentration were used to confirm the physiological status of each donor. Denuded oocytes and their follicular cells were recovered from follicles (n = 675) distributed into 4 types (preantral, small antral ∼0.2–0.39 mm, medium antral ∼0.4–5.9 mm, and large antral ∼6–8 mm). Total RNA was extracted and reverse transcribed, and the levels of expression for these 2 genes were determined using a quantitative real-time polymerase chain reaction technique; the data were evaluated by ANOVA. Relative expressions levels of GDF-9 and BMP-15 transcripts were detected in the oocyte and follicular cells in all follicular stages evaluated, showing differential changes
Lenguagedc.language.isoen
Publisherdc.publisherElsevier Inc.
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceTheriogenology
Keywordsdc.subjectBitch oocyte
Keywordsdc.subjectFollicular development
Keywordsdc.subjectParacrine factors
Keywordsdc.subjectTranscripts
Títulodc.titleTemporal expression of GDF-9 and BMP-15 mRNAs in canine ovarian follicles
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


Files in this item

Icon

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile