Differential human Th22-lymphocyte response triggered by Aggregatibacter actinomycetemcomitans serotypes
Author
dc.contributor.author
Díaz Zúñiga, Jaime
Author
dc.contributor.author
Melgar Rodríguez, Samanta
Author
dc.contributor.author
Monasterio, Gustavo
Author
dc.contributor.author
Pujol, Myriam
Author
dc.contributor.author
Rojas, Leticia
Author
dc.contributor.author
Alvarez, Carla
Author
dc.contributor.author
Carvajal, Paola
Author
dc.contributor.author
Vernal Astudillo, Rolando
Admission date
dc.date.accessioned
2018-12-20T15:25:11Z
Available date
dc.date.available
2018-12-20T15:25:11Z
Publication date
dc.date.issued
2017
Cita de ítem
dc.identifier.citation
Archives of Oral Biology, Volumen 78, 2017, Pages 26-33.
Identifier
dc.identifier.issn
18791506
Identifier
dc.identifier.issn
00039969
Identifier
dc.identifier.other
10.1016/j.archoralbio.2017.02.008
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/159184
Abstract
dc.description.abstract
Objective: In Aggregatibacter actinomycetemcomitans, different serotypes have been described based on
lipopolysaccharide (LPS) antigenicity. When T lymphocytes were stimulated with these serotypes,
different patterns of T-helper (Th)1 and Th17-type of immune responses were reported. Recently, two
new Th phenotypes have been described and named Th9 and Th22 lymphocytes; however, their role in
the pathogenesis of periodontitis remains unclear. This study aimed to investigate the potential Th9 and/
or Th22 lymphocyte responses when stimulated with autologous dendritic cells infected with different A.
actinomycetemcomitans serotypes.
Methods: Monocyte-derived dendritic cells and naïve CD4+ T lymphocytes were obtained from healthy
donors and stimulated with different serotypes of A. actinomycetemcomitans at a multiplicity of infection
MOI = 102 or their purified LPS (10–50 ng/ml). The levels for the Th9 and Th22-associated cytokines, as
well as the transcription factor master-switch genes implied in their differentiation Spi-B and AhR, were
quantified by qPCR and ELISA.
Results: When stimulated with the serotype b of A. actinomycetemcomitans, higher levels of interleukin
(IL)-6 and tumor necrosis factor (TNF)-a were detected in dendritic cells, as well as higher levels of IL-22
and AhR were detected in T lymphocytes, when compared with stimulation with the other serotypes.
Conclusions: The serotype b of A. actinomycetemcomitans has a higher capacity of trigger Th22-type of
immune response in both dendritic cells and T lymphocytes. These data allow us to suggest that, when
the serotype b of A. actinomycetemcomitans is a significant part of the subgingival biofilm, the Th22
polarization might be triggered within the periodontal lesion.