| Author | dc.contributor.author | Toledo, Héctor | |
| Author | dc.contributor.author | Jerez, Carlos A. | |
| Admission date | dc.date.accessioned | 2019-01-29T12:39:47Z | |
| Available date | dc.date.available | 2019-01-29T12:39:47Z | |
| Publication date | dc.date.issued | 1985 | |
| Cita de ítem | dc.identifier.citation | FEBS Letters, Volumen 193, Issue 1, 1985, Pages 17-21 | |
| Identifier | dc.identifier.issn | 00145793 | |
| Identifier | dc.identifier.other | 10.1016/0014-5793(85)80070-3 | |
| Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/159640 | |
| Abstract | dc.description.abstract | The in vitro methylation of the elongation factor EF-Tu from Escherichia coli was investigated. The methylation of newly synthesized EF-Tu was obtained using lambda rifd 18 DNA as template and S-adenosyl [methyl-3H]methionine as methyl donor. About 3 mol methyl residues were incorporated for every 10 mol EF-Tu synthesized. Analysis of the nature of the methyl-containing residues by protein hydrolysis followed by paper chromatography showed that both mono- and dimethyllysine were present. The methylation of EF-Tu was also studied separately from its synthesis by using cell-free systems with artificially undermethylated components. | |
| Lenguage | dc.language.iso | en | |
| Source | dc.source | FEBS Letters | |
| Keywords | dc.subject | DNA-dependent system | |
| Keywords | dc.subject | Elongation factor EF-Tu | |
| Keywords | dc.subject | Ethionine | |
| Keywords | dc.subject | Protein methylation | |
| Título | dc.title | In vitro methylation of the elongation factor EF-Tu from Escherichia coli | |
| Document type | dc.type | Artículo de revista | |
| dcterms.accessRights | dcterms.accessRights | Acceso a solo metadatos | |
| Cataloguer | uchile.catalogador | jmm | |
| Indexation | uchile.index | Artículo de publicación SCOPUS | |
| uchile.cosecha | uchile.cosecha | SI | |
