Author | dc.contributor.author | Ramírez, Eugenio | |
Author | dc.contributor.author | Cartier Rovirosa, Luis | |
Author | dc.contributor.author | Flores, R. | |
Admission date | dc.date.accessioned | 2019-01-29T13:47:46Z | |
Available date | dc.date.available | 2019-01-29T13:47:46Z | |
Publication date | dc.date.issued | 2004 | |
Cita de ítem | dc.identifier.citation | Archives of Virology, Volumen 149, Issue 12, 2004, Pages 2307-2317 | |
Identifier | dc.identifier.issn | 03048608 | |
Identifier | dc.identifier.other | 10.1007/s00705-004-0387-7 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/159807 | |
Abstract | dc.description.abstract | HTLV-1 is the causative agent of HAM/TSP. This neurological disease affects the CNS producing damage of the motor tracts at the spinal cord. The HAM/TSP pathogenesis remains undefined. It could include direct and indirect actions of HTLV-1. We studied the effect of purified HTLV-1 and the PBMC of 22 Chilean patients co-cultivated with fetal neurons of mouse (CNh cells): 8 HAM/TSP, 8 HTLV-1 carriers, and 6 non-infected controls. The viral antigens and provirus in CNh cells was evaluated with monoclonal and polyclonal antibodies reacting with HTLV-1 by immunofluorescence assay and PCR at 0, 7 and 15 days of co-cultures, respectively. Viral antigens were detected in 0.1-0.5%, and 0-0.3% of the neurons incubated with lymphocytes of HAM/TSP patients and HTLV-1 carriers, respectively. Neurons incubated with cells of 7 HAM/TSP patients, and 3 HTLV-1 carriers showed the presence of nucleotide sequences of tax gene. These results would be showing that CNh cells would express viral antigens and provirus. The HTLV-1 or their proteins were capable in vitro to produce structural and growth changes in the cytoskeleton of CNh neurons. In this series, the purified HTLV-1 was more effective in the neural changes than PBMC of HAM/TSP or HTLV-1 carriers. | |
Lenguage | dc.language.iso | en | |
Source | dc.source | Archives of Virology | |
Keywords | dc.subject | Genetics | |
Keywords | dc.subject | Applied Microbiology and Biotechnology | |
Título | dc.title | In vitro cytoskeleton changes of mouse neurons induced by purified HTLV-1, and PBMC from HAM/TSP patients and HTLV-1 carriers | |
Document type | dc.type | Artículo de revista | |
dcterms.accessRights | dcterms.accessRights | Acceso a solo metadatos | |
Cataloguer | uchile.catalogador | jmm | |
Indexation | uchile.index | Artículo de publicación SCOPUS | |
uchile.cosecha | uchile.cosecha | SI | |