Cyclovirus in nasopharyngeal aspirates of Chilean children with respiratory infections
Author
dc.contributor.author
Phan, Tung Gia
Author
dc.contributor.author
Luchsinger Farías, Vivian
Author
dc.contributor.author
Avendaño Carvajal, Luis
Author
dc.contributor.author
Deng, Xutao
Author
dc.contributor.author
Delwart, Eric
Admission date
dc.date.accessioned
2019-01-29T13:56:15Z
Available date
dc.date.available
2019-01-29T13:56:15Z
Publication date
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2014
Cita de ítem
dc.identifier.citation
Journal of General Virology (2014), 95, 922–927
Identifier
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14652099
Identifier
dc.identifier.issn
00221317
Identifier
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10.1099/vir.0.061143-0
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/160108
Abstract
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Some respiratory tract infections remain unexplained despite extensive testing for common pathogens. Nasopharyngeal aspirates (NPAs) from 120 Chilean infants from Santiago with acute lower respiratory tract infections were analysed by viral metagenomics, revealing the presence of nucleic acids from anelloviruses, adenovirus-associated virus and 12 known respiratory viral pathogens. A single sequence read showed translated protein similarity to cycloviruses. We used inverse PCR to amplify the complete circular ssDNA genome of a novel cyclovirus we named CyCV-ChileNPA1. Closely related variants were detected using PCR in the NPAs of three other affected children that also contained anelloviruses. This report increases the current knowledge of the genetic diversity of cycloviruses whose detection in multiple NPAs may reflect a tropism for human respiratory tissues.