Calcium-activated chloride currents and non-selective cation channels in a novel cystic fibrosis-derived human pancreatic duct cell line
Author
dc.contributor.author
Eguiguren, Ana Luisa
Author
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Ríos, Juan
Author
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Riveros, Nora
Author
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Sepúlveda, Francisco V.
Author
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Stutzin Schottlander, Andrés
Admission date
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2019-01-29T14:14:00Z
Available date
dc.date.available
2019-01-29T14:14:00Z
Publication date
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1996
Cita de ítem
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Biochemical and Biophysical Research Communications, Volumen 225, Issue 2, 2018, Pages 505-513
Identifier
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0006291X
Identifier
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10.1006/bbrc.1996.1203
Identifier
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https://repositorio.uchile.cl/handle/2250/160294
Abstract
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The modulation of ion fluxes across the plasma membrane of epithelial cells is central for fluid secretion and absorption. Their disruption can lead to pathological states. An example is cystic fibrosis (CF), a disease characterized by abnormal functioning of the cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-modulated chloride channel. Here we report the characterization of calcium-activated, DIDS sensitive chloride current and non-selective calcium-activated cation channels in a novel human pancreatic duct cell line (YHV-1) derived from a non-ΔF508 mutation CF patient bearing a severe phenotype. Southern blot analysis of the CFTR gene indicates a distinct electrophoretic pattern for the region spanned by exons 15-24, a result presumably related to a mutation which has yet to be identified. In contrast to large calcium-activated chloride currents there were no cAMP-dependent CFTR-type chloride currents. Non-selective cation channels were blocked by intracellular AT