Effect of S-adenosylmethionine on human rotavirus RNA synthesis
Author
dc.contributor.author
Spencer, Nicolás
Author
dc.contributor.author
GARCIA,
Admission date
dc.date.accessioned
2019-01-29T14:47:00Z
Available date
dc.date.available
2019-01-29T14:47:00Z
Publication date
dc.date.issued
1984
Cita de ítem
dc.identifier.citation
Journal of Virology, Volumen 52, Issue 1, 2018, Pages 188-197
Identifier
dc.identifier.issn
0022538X
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/160518
Abstract
dc.description.abstract
The characteristics of human rotavirus-associated RNA polymerase activity have been examined in relation to the effects of ribonucleoside triphosphate analogs and S-adenosylmethionine. These effects were analyzed by testing two forms of activated virus particles: EDTA- and heat-treated virions. The former lack outer shell proteins, and activation by means of heat treatment does not introduce any apparent modification in virion structure. Virus-associated RNA polymerase shows similar properties in both preparations, suggesting that outer proteins are not directly involved in RNA synthesis. Transcription in this virus is specifically dependent on a hydrolyzable form of ATP. Such a requirement is not overcome by preincubation or by the addition of S-adenosylmethionine, suggesting a hypothetical mechanism that couples transcription to ATP hydrolysis. The addition of S-adenosylmethionine stimulated transcription and diminished the K(m) value not only for ATP but also for the other three rib