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Authordc.contributor.authorBehrens Pellegrino, María Isabel 
Authordc.contributor.authorOberhauser, Andres 
Authordc.contributor.authorBezanilla, Francisco 
Authordc.contributor.authorLatorre, Ramón 
Cita de ítemdc.identifier.citationJournal of General Physiology, Volumen 93, Issue 1, 2018, Pages 23-41
Abstractdc.description.abstractSquid optic nerve sodium channels were characterized in planar bilayers in the presence of batrachotoxin (BTX). The channel exhibits a conductance of 20 pS in symmetrical 200 mM NaCI and behaves as a sodium electrode. The single-channel conductance saturates with increasing the concentration of sodium and the channel conductance vs. sodium concentration relation is well described by a simple rectangular hyperbola. The apparent dissociation constant of the channel for sodium is 11 mM and the maximal conductance is 23 pS. The selectivity determined from reversal potentials obtained in mixed ionic conditions is Na+ -Li+ > K+ > Rb+ > Cs+ Calcium blocks the channel in a voltage-dependent manner. Analysis of single-channel membranes showed that the probability of being open (P0) vs. voltage relation is sigmoidal with a value of 0.5 between -90 and -100 mV. The fitting of P0 requires at least two closed and one open state. The apparent gating charge required to move through the whole transmem
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
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Sourcedc.sourceJournal of General Physiology
Títulodc.titleBatrachotoxin-modified sodium channels from squid optic nerve in planar bilayers: Ion conduction and gating properties
Document typedc.typeArtículo de revista
Indexationuchile.indexArtículo de publicación SCOPUS

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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile