Certain large forms of circulating immunoreactive human growth hormone are in fact immunoglobulins
Author
dc.contributor.author
Campino, Carmen
Author
dc.contributor.author
Szecowka, Jaroslaw
Author
dc.contributor.author
Michelsen, Harold
Author
dc.contributor.author
Serón Ferré, María
Admission date
dc.date.accessioned
2019-01-29T14:49:19Z
Available date
dc.date.available
2019-01-29T14:49:19Z
Publication date
dc.date.issued
1990
Cita de ítem
dc.identifier.citation
Journal of Clinical Endocrinology and Metabolism, Volumen 70, Issue 3, 2018, Pages 601-605
Identifier
dc.identifier.issn
19457197
Identifier
dc.identifier.issn
0021972X
Identifier
dc.identifier.other
10.1210/jcem-70-3-601
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/160814
Abstract
dc.description.abstract
To explain frequent discordances between serum GH levels and clinical manifestation of acromegaly, we investigated the possibility that certain immunoglobulins G (IgGs) might be responsible for the displacement of [125I]human (h) GH in the hGH RIA. We incubated dilute sera from seven active acromegalics (basal immunoreactive hGH, 22–313 μg/L) with rat adipocyte plasma membranes adsorbed on polystyrene plates. IgGs that bound to GH receptor sites in the absence and presence of 250 nM hGH (for nonspecific binding) were detected using anti-hlgG (Fc-specific) antibody conjugated with alkaline phosphatase. In this system two of the seven sera studied tested positive for IgGs against GH-binding sites (serum 4 in 1:400 dilution, and serum 7 in 1:10 dilution). We studied further the serum with the highest titer. On Sephadex G-100, most of the GH-like immunoreactivity (assayed by RIA) present in serum 4 coeluted with IgGs (assayed by immunodiffussion) as a high mol wt (≥ 150 kDa) component. To