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Authordc.contributor.authorCaviedes, Pablo 
Authordc.contributor.authorOlivares, Enrique 
Authordc.contributor.authorSalas, Karime 
Authordc.contributor.authorCaviedes, Raúl 
Authordc.contributor.authorJaimovich Pérez, Enrique 
Cita de ítemdc.identifier.citationJournal of Molecular and Cellular Cardiology, Volumen 25, Issue 7, 2018, Pages 829-845
Abstractdc.description.abstractA cell line (RCVC) in permanent culture was developed from adult rat ventricular cells; transformation was attained by incubation with conditioned media from UCHTI, a rat thyroid cell line. Immortalized ventricular cells have a doubling time of 20 h, contact inhibition of growth, and display some muscle markers such as a high glycogen content and positive immunoreaction for myoglobin, α-sarcomeric actin, α-actin and desmin. A microsomal fraction from these cells was shown to bind 3H-nitrendipine with a maximal capacity of 295 fmol/mg protein and an equilibrium dissociation constant of 0.7 nM. Nifedipine-sensitive 45Ca2+ influx was evident in partially depolarized cells (40 mM K+ in the incubation medium). An equivalent influx, induced by the calcium channel agonist BAYK-8644 and CGP-28392, was obtained in normally polarized cells. Patch clamp studies show slow inward currents that can be completely blocked by 5 μM nifedipine; cells were induced to further differentiation by culturing i
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.uri
Sourcedc.sourceJournal of Molecular and Cellular Cardiology
Keywordsdc.subjectCalcium channels
Keywordsdc.subjectCalcium flux
Keywordsdc.subjectCardiac cell line
Keywordsdc.subjectCell transformation
Keywordsdc.subjectCultured heart muscle
Keywordsdc.subjectDihydropyridine receptors
Keywordsdc.subjectIon channels
Títulodc.titleCalcium Fluxes, Ion Currents and Dihydropyridine Receptors in a New Immortal Cell Line from Rat Heart Muscle
Document typedc.typeArtículo de revista
Indexationuchile.indexArtículo de publicación SCOPUS

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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile