Metabolism of acetaldehyde by rat isolated aortic rings: Does endothelial tissue contribute to its extrahepatic metabolism?
Author
dc.contributor.author
Tampier de Jong, Lutske
Author
dc.contributor.author
Cariz, Segundo
Author
dc.contributor.author
Quintanilla González, María Elena
Admission date
dc.date.accessioned
2019-01-29T14:51:21Z
Available date
dc.date.available
2019-01-29T14:51:21Z
Publication date
dc.date.issued
1993
Cita de ítem
dc.identifier.citation
Alcohol, Volumen 10, Issue 3, 2018, Pages 203-206
Identifier
dc.identifier.issn
07418329
Identifier
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10.1016/0741-8329(93)90036-N
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/161006
Abstract
dc.description.abstract
Acetaldehyde (AcH) metabolism in isolated aortic rings was studied by assessing in vitro-added AcH disappearing rate by head space gas chromatography. It was found that AcH was metabolized by aortic rings or by homogenates prepared in 0.1 M phosphate buffer containing Triton X-100, by an NAD-dependent enzyme with characteristics similar to those of aldehyde dehydrogenase (AIDH) present in mitochondria from rat liver and brain. This enzyme appears to be present in the vascular endothelium, since the action of aortic rings showed a remarkable decrease by its removal. Extrahepatic metabolism of AcH was assessed by the differences between AcH levels found in samples of blood obtained from the suprahepatic vein, carotid artery, femoral vein, and tail cut of rats. The in vitro activity of aortic rings, as well as the extrahepatic AcH metabolism, were significantly decreased by pretreatment of rats with disulfiram. The wide distribution of vascular endothelium throughout the body suggests tha