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Authordc.contributor.authorNaranjo, J. 
Authordc.contributor.authorLatorre, Claudio 
Admission datedc.date.accessioned2019-01-29T14:51:22Z
Available datedc.date.available2019-01-29T14:51:22Z
Publication datedc.date.issued1993
Cita de ítemdc.identifier.citationBiophysical Journal, Volumen 64, Issue 4, 2018, Pages 1038-1050
Identifierdc.identifier.issn00063495
Identifierdc.identifier.other10.1016/S0006-3495(93)81469-3
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/161011
Abstractdc.description.abstractBatrachotoxin-modified Na+ channels from toad muscle were inserted into planar lipid bilayers composed of neutral phospholipids. Single-channel conductances were measured for [Na+] ranging between 0.4 mM and 3 M. When membrane preparations were made in the absence of protease inhibitors, two open conductance states were identified: a fully open state (16.6 pS in 200 mM symmetrical NaCl) and a substate that was 71% of the full conductance. The substate was predominant at [Na+] > 65 mM, whereas the presence of the fully open state was predominant at [Na+] < 15 mM. Addition of protease inhibitors during membrane preparation stabilized the fully open state over the full range of [Na+] studied. In symmetrical Na+ solutions and in biionic conditions, the ratio of amplitudes remained constant and the two open states exhibited the same permeability ratios of PLi/PNa and PCs/PNa. The current-voltage relations for both states showed inward rectification only at [Na+] < 10 mM, suggesting the pres
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceBiophysical Journal
Keywordsdc.subjectBiophysics
Títulodc.titleIon conduction in substates of the batrachotoxin-modified Na+ channel from toad skeletal muscle
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile