Lipopolysaccharide-independent radioimmunoprecipitation and identification of structural and in vivo induced immunogenic surface proteins of Salmonella typhi in typhoid fever
Author
dc.contributor.author
Aron, Lieselotte
Author
dc.contributor.author
Faundez, Gustavo
Author
dc.contributor.author
Gonzalez, Carlos
Author
dc.contributor.author
Roessler, Emilio
Author
dc.contributor.author
Cabello, Felipe
Admission date
dc.date.accessioned
2019-01-29T14:52:55Z
Available date
dc.date.available
2019-01-29T14:52:55Z
Publication date
dc.date.issued
1993
Cita de ítem
dc.identifier.citation
Vaccine, Volumen 11, Issue 1, 2018, Pages 10-17
Identifier
dc.identifier.issn
0264410X
Identifier
dc.identifier.other
10.1016/0264-410X(93)90334-T
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/161125
Abstract
dc.description.abstract
The humoral response to Salmonella typhi is important for protective immunity against typhoid fever, as indicated by the protection obtained with killed cell vaccines and component vaccines (outer membrane proteins, Vi antigen) in animals and human beings. Nonetheless, analysis and interpretation of host humoral immune response to S. typhi surface antigens have been difficult because of the complex structure of the S. typhi envelope and the lack of purified reagents for detection of immune response to individual surface components. Normal and convalescent human sera from typhoid fever patients were absorbed with S. typhi lipopolysaccharide. These sera were used in radioimmunoprecipitation assays of whole S. typhi cells and S. typhi membranes labelled with either 125I or 35S-methionine. This strategy has permitted the unequivocal identification of a humoral immune response to structural and in vivo induced outer membrane proteins of S. typhi. In this manner, we have identified the porin
Lipopolysaccharide-independent radioimmunoprecipitation and identification of structural and in vivo induced immunogenic surface proteins of Salmonella typhi in typhoid fever