Properties of the Ryanodine‐sensitive Release Channels that Underlie Caffeine‐induced Ca2+ Mobilization from Intracellular Stores in Mammalian Sympathetic Neurons
Author
dc.contributor.author
Hernández‐Cruz, A.
Author
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Díaz‐Muñoz, M.
Author
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Gómez‐Chavarín, M.
Author
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Canñedo‐Merino, R.
Author
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Protti, D. A.
Author
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Escobar, A. L.
Author
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Sierralta, J.
Author
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Suárez‐Isla, B. A.
Admission date
dc.date.accessioned
2019-01-29T14:55:17Z
Available date
dc.date.available
2019-01-29T14:55:17Z
Publication date
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1995
Cita de ítem
dc.identifier.citation
European Journal of Neuroscience, Volumen 7, Issue 8, 2018, Pages 1684-1699
Identifier
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14609568
Identifier
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0953816X
Identifier
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10.1111/j.1460-9568.1995.tb00690.x
Identifier
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https://repositorio.uchile.cl/handle/2250/161328
Abstract
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The most compelling evidence for a functional role of caffeine‐sensitive intracellular Ca2+ reservoirs in nerve cells derives from experiments on peripheral neurons. However, the properties of their ryanodine receptor calcium release channels have not been studied. This work combines single‐cell fura‐2 microfluorometry, [3 H]ryanodine binding and recording of Ca2+ release channels to examine calcium release from these intracellular stores in rat sympathetic neurons from the superior cervical ganglion. Intracellular Ca2+ measurements showed that these cells possess caffeine‐sensitive intracellular Ca2+ stores capable of releasing the equivalent of 40% of the calcium that enters through voltage‐gated calcium channels. The efficiency of caffeine in releasing Ca2+ showed a complex dependence on [Ca2+]i. Transient elevations of [Ca2+]i by 50–500 nM were facilitatory, but they became less facilitatory or depressing when [Ca2+]i reached higher levels. The caffeine‐induced Ca2+ release and its
Properties of the Ryanodine‐sensitive Release Channels that Underlie Caffeine‐induced Ca2+ Mobilization from Intracellular Stores in Mammalian Sympathetic Neurons