The Catalytic Subunit of cAMP‐Dependent Protein Kinase from Ascaris suum The Cloning and Structure of a Novel Subtype of Protein Kinase A
Author
dc.contributor.author
Jung, Stephan
Author
dc.contributor.author
Hoffmann, Ralf
Author
dc.contributor.author
Rodriguez, Patricio H.
Author
dc.contributor.author
Mutzel, Rupert
Author
dc.contributor.author
Hofer, Hans Werner
Admission date
dc.date.accessioned
2019-01-29T14:55:21Z
Available date
dc.date.available
2019-01-29T14:55:21Z
Publication date
dc.date.issued
1995
Cita de ítem
dc.identifier.citation
European Journal of Biochemistry, Volumen 232, Issue 1, 2018, Pages 111-117
Identifier
dc.identifier.issn
14321033
Identifier
dc.identifier.issn
00142956
Identifier
dc.identifier.other
10.1111/j.1432-1033.1995.tb20788.x
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/161344
Abstract
dc.description.abstract
A complete cDNA clone encoding the catalytic subunit of cAMP‐dependent protein kinase of Ascaris suum was constructed from two overlapping partial clones. The encoded sequence of 337 ammo acids is 48 % identical with the sequence of mouse Cα subunit. Approximately the same low similarity was found with the sequence of the C subunit from another nematode, Caenorhabditis elegans. The N‐terminal 14 amino acids and the myristoylation site of the mammalian protein are not contained in the enzyme from Ascaris. Two cysteines (Cys33 and Cys319) replace a basic residue in the N‐terminal region and an acidic amino acid near the C‐terminus which are conserved in all known C subunits from other sources. The substitutions provide the possibility of disulfide bridge formation between the N‐terminal and C‐terminal parts of the protein. There is strong evidence that a single gene encodes cAMP‐dependent protein kinase in Ascaris. Modelling of the sequence into the coordinates of the X‐ray structure of