Comparison of cartilage histopathology assessment systems on human knee joints at all stages of osteoarthritis development

Abstract

Objective: To compare the MANKIN and OARSI cartilage histopathology assessment systems using human articular cartilage from a large number of donors across the adult age spectrum representing all levels of cartilage degradation. Design: Human knees (n= 125 from 65 donors; age range 23-92) were obtained from tissue banks. All cartilage surfaces were macroscopically graded. Osteochondral slabs representing the entire central regions of both femoral condyles, tibial plateaus, and the patella were processed for histology and Safranin O - Fast Green staining. Slides representing normal, aged, and osteoarthritis (OA) tissue were scanned and electronic images were scored online by five observers. Statistical analysis was performed for inter- and intra-observer variability, reproducibility and reliability. Results: The inter-observer variability among five observers for the MANKIN system showed a similar good Intra-class correlation coefficient (ICC > 0.81) as for the OARSI system (ICC > 0.78)