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Authordc.contributor.authorVivar, Raúl 
Authordc.contributor.authorHumeres, Claudio 
Authordc.contributor.authorVarela, Marcelo 
Authordc.contributor.authorAyala, Pedro 
Authordc.contributor.authorGuzmán, Nancy 
Authordc.contributor.authorOlmedo, Ivonne 
Authordc.contributor.authorCatalán, Mabel 
Authordc.contributor.authorBoza Fuentes, Pía 
Authordc.contributor.authorMuñoz, Claudia 
Authordc.contributor.authorDíaz Araya, Guillermo 
Admission datedc.date.accessioned2019-01-29T15:36:43Z
Available datedc.date.available2019-01-29T15:36:43Z
Publication datedc.date.issued2012
Cita de ítemdc.identifier.citationExperimental and Molecular Pathology, Volumen 93, Issue 1, 2018, Pages 1-7
Identifierdc.identifier.issn00144800
Identifierdc.identifier.issn10960945
Identifierdc.identifier.other10.1016/j.yexmp.2012.01.010
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/161829
Abstractdc.description.abstractCardiac fibroblast (CF) death by ischemia/reperfusion (I/R) has major implications for cardiac wound healing. Although IGF-1 has well-known cytoprotective effects, no study has been done on CF subjected to simulated I/R.Simulated ischemia of neonate rat CF was performed in a free oxygen chamber in an ischemic medium; reperfusion was done in normal culture conditions. Cell viability was evaluated by trypan blue assay, and apoptosis by a FACS flow cytometer; p-ERK-1/2 and p-Akt levels were determined by western blot.We showed that simulated I/R triggers CF death by necrosis and apoptosis. IGF-1 partially inhibits I/R-induced apoptosis. PD98059 and LY294002 neutralize the preventive effects of IGF-1.Conclusion: IGF-1 partially inhibits CF apoptosis induced by simulated I/R by PI3K/Akt- and MEK/ERK1/2-dependent signaling pathways. © 2012 Elsevier Inc.
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceExperimental and Molecular Pathology
Keywordsdc.subjectApoptosis
Keywordsdc.subjectCardiac fibroblast
Keywordsdc.subjectIGF-1
Keywordsdc.subjectIschemia/reperfusion
Títulodc.titleCardiac fibroblast death by ischemia/reperfusion is partially inhibited by IGF-1 through both PI3K/Akt and MEK-ERK pathways
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile