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Authordc.contributor.authorRosemblatt Silber, Mario César 
Authordc.contributor.authorPérez, Gonzalo 
Authordc.contributor.authorJaimovich Pérez, Enrique 
Admission datedc.date.accessioned2019-01-29T15:47:29Z
Available datedc.date.available2019-01-29T15:47:29Z
Publication datedc.date.issued1991
Cita de ítemdc.identifier.citationMolecular and Cellular Biochemistry, Volumen 106, Issue 2, 2018, Pages 99-107
Identifierdc.identifier.issn03008177
Identifierdc.identifier.issn15734919
Identifierdc.identifier.other10.1007/BF00230175
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/162375
Abstractdc.description.abstractWe have established several hybridoma lines that produce monoclonal antibodies against transverse tubule (t-tubule) proteins from frog skeletal muscle. The specificity of these antibodies was characterized by ELISA and Western immunoblotting with purified t-tubule, sarcoplasmic reticulum and partially purified sarcolemmal membranes. One of the monoclonal antibodies (2/34.4) recognizes a band of 109 000 Da in immunoblots. When purified frog t-tubule vesicles were preincubated with this antibody we observed an increase in the rate of the Mg2+-ATPase enzyme (up to six fold) which was dependent on antibody concentration. Immunocytological experiments done on cryostat sections of frog muscle indicate that the antigen recognized by this antibody is localized mainly at the level of the t-tubules (I band) and to a lesser extent at the sarcolemma. These results indicate that monoclonal antibody 2/34.4 recognizes the t-tubule Mg2+-ATPase and modulates its activity. This antibody should be useful
Lenguagedc.language.isoen
Publisherdc.publisherKluwer Academic Publishers
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceMolecular and Cellular Biochemistry
Keywordsdc.subjectfrog skeletal muscle
Keywordsdc.subjectMg2+-ATPase
Keywordsdc.subjectmonoclonal antibodies
Keywordsdc.subjecttransverse tubules
Títulodc.titleModulation of the activity of the transverse tubule Mg2+ATPase from frog skeletal muscle by a monoclonal antibody in vitro
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile