Show simple item record

Authordc.contributor.authorNunez, Sarah 
Authordc.contributor.authorAlvarez Alvarez, Miguel Adrián 
Authordc.contributor.authorSmith, Pamela 
Authordc.contributor.authorTapia Tapia, Alejandra 
Authordc.contributor.authorGlass, Larry 
Admission datedc.date.accessioned2019-01-29T15:50:04Z
Available datedc.date.available2019-01-29T15:50:04Z
Publication datedc.date.issued1994
Cita de ítemdc.identifier.citationAmerican Journal of Physiology - Cell Physiology, Volumen 267, Issue 6 36-6, 2018,
Identifierdc.identifier.issn03636143
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/162546
Abstractdc.description.abstractCaco-2 cells were used as a model of human intestinal epithelium to investigate the role of redox systems in transepithelial transport of 59Fe3+. The cells reduced Fe3+ present in the apical medium; the reduction was 50% inhibited by adriamycin and p-chloromercuribenzoate. Addition of [14C]ascorbate to the basolateral medium resulted in accumulation of 14C radioactivity in both cells and apical medium; apical radioactivity increased with time and was probably caused by paracellular flux. The cells provided Fe3+ reduction capacity to the apical incubation medium. Addition of ascorbate to the basolateral medium increased this reduc- tion capacity 2-fold and the cellular uptake of 59Fe3+ 1.8-fold. Adriamycin significantly inhibited both cellular 59Fe uptake and Fe transport into the basolateral side. The results indicate that Caco-2 cells reduce apical Fe3+ by two parallel mechanisms: by a plasma membrane ferrireductase and by the secretion of reductants of either cellular or basolateral
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceAmerican Journal of Physiology - Cell Physiology
Keywordsdc.subjectascorbate
Keywordsdc.subjectCaco-2
Keywordsdc.subjectepithelium
Keywordsdc.subjectferrireductase
Keywordsdc.subjectintestine
Keywordsdc.subjectiron absorption
Keywordsdc.subjectiron reduction
Keywordsdc.subjectiron transport
Títulodc.titleRole of redox systems on Fe3+ uptake by transformed human intestinal epithelial (Caco-2) cells
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


Files in this item

Icon

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile