Changes in IP 3 metabolism during skeletal muscle development in vivo and in vitro
Author
dc.contributor.author
Carrasco, María Angélica
Author
dc.contributor.author
Marambio, Paola
Author
dc.contributor.author
Jaimovich Pérez, Enrique
Admission date
dc.date.accessioned
2019-01-29T15:54:50Z
Available date
dc.date.available
2019-01-29T15:54:50Z
Publication date
dc.date.issued
1997
Cita de ítem
dc.identifier.citation
Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Volumen 116, Issue 2, 2018, Pages 173-181
Identifier
dc.identifier.issn
03050491
Identifier
dc.identifier.other
10.1016/S0305-0491(96)00244-1
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/162722
Abstract
dc.description.abstract
We have investigated whether IP 3 metabolism presents particular changes during critical stages of muscle development. With this aim, we have measured IP 3 formation through phospholipase C activity, IP 3 removal through IP 3 5-phosphatase and IP 3 3-kinase activities, as well as IP 3 mass, during myogenesis in vivo and in vitro. In developing rat skeletal muscle, both IP 3 3-kinase and 5-phosphatase activities were relatively constant from embryonary day 15, the earliest age studied, to postnatal day 10; 5-phosphatase decreased upon further development. A transient, major increase in phospholipase C activity was evident at embryonary day 18 while a non-significant increase in IP 3 mass was detected at this embrionary age. In rat skeletal muscle in primary culture, all enzyme activities as well as the mass of IP 3 increased significantly in myotubes compared to myoblasts. Myotubes incubated with calcitonin gene-related peptide, responded with a transient increase in IP 3 mass after 2 t