Outwardly rectifying Cl - channel in guinea pig small intestinal villus enterocytes: Effect of inhibitors
Author
dc.contributor.author
Monaghan, Alan S.
Author
dc.contributor.author
Mintenig, Gerard M.
Author
dc.contributor.author
Sepúlveda, Francisco V.
Admission date
dc.date.accessioned
2019-01-29T15:54:53Z
Available date
dc.date.available
2019-01-29T15:54:53Z
Publication date
dc.date.issued
1997
Cita de ítem
dc.identifier.citation
American Journal of Physiology - Gastrointestinal and Liver Physiology, Volumen 273, Issue 5 36-5, 2018,
Identifier
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01931857
Identifier
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https://repositorio.uchile.cl/handle/2250/162731
Abstract
dc.description.abstract
Previous studies in enterocytes isolated from the villus region of small intestinal epithelium have identified a macroscopic current carried by Cl. In this work a single-channel patch-clamp study was carried out in the same cells, and a spontaneously active, outwardly rectifying Cl channel was identified and proposed to underlie the whole cell current. The channel had conductances of 62 and 19 pS at 80 and -80 mV, respectively, in symmetrical Cl solutions in excised patches. Similar activity was seen in cell-attached patches, but only outward currents could be discerned in this configuration. The activity of the channel, measured as open probability, was independent of intracellular calcium levels and voltage. The selectivity sequence for different anions was SCN > I > Br > CI > F > (gluconate, glutamate, SO 4/ 2). The channel was inhibited by 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), verapamil, and 4-hydroxytamoxifen (but not by tamoxifen), with potencies similar to those ob