Arachidonic acid release from rat Leydig cells depends on the presence of luteinizing hormone/human chorionic gonadotrophin receptors
Author
dc.contributor.author
Moraga,
Author
dc.contributor.author
Llanos Silva, Miguel
Author
dc.contributor.author
Ronco Macchiavello, Ana María
Admission date
dc.date.accessioned
2019-01-29T15:54:57Z
Available date
dc.date.available
2019-01-29T15:54:57Z
Publication date
dc.date.issued
1997
Cita de ítem
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Journal of Endocrinology, Volumen 154, Issue 2, 2018, Pages 201-209
Identifier
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00220795
Identifier
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10.1677/joe.0.1540201
Identifier
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https://repositorio.uchile.cl/handle/2250/162742
Abstract
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In this work, the involvement of arachidonic acid (AA) in the luteinizing hormone and human chorionic gonadotrophin (LH/hCG) action on Leydig cells was studied. Experiments were first designed to evaluate [14C]AA incorporation into membrane phospholipids. Subsequently, time- course, pulse-chase and dose-response studies of the effect of hCG on [14C]AA release were performed. Results indicated that 4 h was optimal for maximal incorporation of [14C]AA into membrane phospholipids of viable Leydig cells. Pulse-chase experiments and studies performed to evaluate the effect of different doses of hCG on [14C]AA release demonstrated that this hornlone stimulates [14C]AA release in a dose-response and time-dependent manner. Furthermore, using a desensitised animal model, a link between the presence of LH/hCG receptors and LH/hCG-stimulated [14C]AA release in Leydig cells could be established. In fact, the amount of [14C]AA released was significantly dependent on, and directly proportional to, t