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Authordc.contributor.authorFoncea, Rocío 
Authordc.contributor.authorAndersson, Monica 
Authordc.contributor.authorKetterman, Albert 
Authordc.contributor.authorBlakesley, Vicky 
Authordc.contributor.authorSapag Hagar, Mario 
Authordc.contributor.authorSugden, Peter H. 
Authordc.contributor.authorLeRoith, Derek 
Authordc.contributor.authorLavandero González, Sergio 
Admission datedc.date.accessioned2019-01-29T15:54:59Z
Available datedc.date.available2019-01-29T15:54:59Z
Publication datedc.date.issued1997
Cita de ítemdc.identifier.citationJournal of Biological Chemistry, Volumen 272, Issue 31, 2018, Pages 19115-19124
Identifierdc.identifier.issn00219258
Identifierdc.identifier.other10.1074/jbc.272.31.19115
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/162751
Abstractdc.description.abstractIn response to insulin-like growth factor-I (IGF-I), neonatal rat cardiac myocytes exhibit a hypertrophic response. The elucidation of the IGF- I signal transduction system in these cells remains unknown. We show here that cardiac myocytes present a single class of high affinity receptors (12,446 ± 3,669 binding sites/cell) with a dissociation constant of 0.36 ± 0.10 nM. Two different β-subunits of IGF-I receptor were detected, and their autophosphorylation was followed by increases in the phosphetyrosine content of extracellular signal-regulated kinases (ERKs), insulin receptor substrate 1, phospholipase C-γ1, and phosphatidylinositol 3-kinase. IGF.I transiently activates c-Raf in cultured neonatal cardiac myocytes, whereas A-raf is activated much less than c-Raf. Two peaks of ERK activity (ERK1 and ERK2) were resolved in cardiac myocytes treated with IGF-I by fast protein liquid chromatography, both being stimulated by IGF-I (with EC50 values for the stimulation of ERK1 and ERK2 by I
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceJournal of Biological Chemistry
Keywordsdc.subjectBiochemistry
Keywordsdc.subjectMolecular Biology
Keywordsdc.subjectCell Biology
Títulodc.titleInsulin-like growth factor-I rapidly activates multiple signal transduction pathways in cultured rat cardiac myocytes
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile