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Authordc.contributor.authorFoncea, Rocío 
Authordc.contributor.authorAndersson, Monica 
Authordc.contributor.authorKetterman, Albert 
Authordc.contributor.authorBlakesley, Vicky 
Authordc.contributor.authorSapag Hagar, Mario 
Authordc.contributor.authorSugden, Peter H. 
Authordc.contributor.authorLeRoith, Derek 
Authordc.contributor.authorLavandero González, Sergio 
Cita de ítemdc.identifier.citationJournal of Biological Chemistry, Volumen 272, Issue 31, 2018, Pages 19115-19124
Abstractdc.description.abstractIn response to insulin-like growth factor-I (IGF-I), neonatal rat cardiac myocytes exhibit a hypertrophic response. The elucidation of the IGF- I signal transduction system in these cells remains unknown. We show here that cardiac myocytes present a single class of high affinity receptors (12,446 ± 3,669 binding sites/cell) with a dissociation constant of 0.36 ± 0.10 nM. Two different β-subunits of IGF-I receptor were detected, and their autophosphorylation was followed by increases in the phosphetyrosine content of extracellular signal-regulated kinases (ERKs), insulin receptor substrate 1, phospholipase C-γ1, and phosphatidylinositol 3-kinase. IGF.I transiently activates c-Raf in cultured neonatal cardiac myocytes, whereas A-raf is activated much less than c-Raf. Two peaks of ERK activity (ERK1 and ERK2) were resolved in cardiac myocytes treated with IGF-I by fast protein liquid chromatography, both being stimulated by IGF-I (with EC50 values for the stimulation of ERK1 and ERK2 by I
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.uri
Sourcedc.sourceJournal of Biological Chemistry
Keywordsdc.subjectMolecular Biology
Keywordsdc.subjectCell Biology
Títulodc.titleInsulin-like growth factor-I rapidly activates multiple signal transduction pathways in cultured rat cardiac myocytes
Document typedc.typeArtículo de revista
Indexationuchile.indexArtículo de publicación SCOPUS

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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile