Cloning and expression in Escherichia coli of genetic determinants for production of and immunity to microcin E492 from Klebsiella pneumoniae
Author
dc.contributor.author
Wilkens, Marcela
Author
dc.contributor.author
Villanueva, Jorge E.
Author
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Cofré, Jaime
Author
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Chnaiderman, Jonás
Author
dc.contributor.author
Lagos Mónaco, Rosalba
Admission date
dc.date.accessioned
2019-01-29T15:55:00Z
Available date
dc.date.available
2019-01-29T15:55:00Z
Publication date
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1997
Cita de ítem
dc.identifier.citation
Journal of Bacteriology, Volumen 179, Issue 15, 2018, Pages 4789-4794
Identifier
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00219193
Identifier
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10.1128/jb.179.15.4789-4794.1997
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/162754
Abstract
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Microcin E492 is a polypeptide antibiotic that is produced and excreted by Klebsiella pneumoniae RYC492. The genetic determinants for microcin synthesis and immunity were cloned in Escherichia coli VCS257 into the cosmid vector pHC79, starting from total DNA of K. pneumoniae RYC492. The microtin E492 expressed in E. coli had the same properties as that of K. pneumoniae, i.e., the same molecular weight, the ability to form ionic channels in planar phospholipid bilayers, and essentially identical biological properties. Microcin E492 expression in E. coli, like that in K. pneumoniae, was mainly in the exponential phase of growth, declining in the stationary phase. The immunity determinant was subcloned into the same vector, and its expression was found to disappear in the stationary phase. This phenomenon is not dependent on rpoS, the stationary-phase sigma factor.