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Authordc.contributor.authorKukuljan Padilla, Manuel 
Authordc.contributor.authorVergara, Leoncio 
Authordc.contributor.authorStojilkovic, Stanko S. 
Admission datedc.date.accessioned2019-01-29T15:55:05Z
Available datedc.date.available2019-01-29T15:55:05Z
Publication datedc.date.issued1997
Cita de ítemdc.identifier.citationBiophysical Journal, Volumen 72, Issue 2 I, 2018, Pages 698-707
Identifierdc.identifier.issn00063495
Identifierdc.identifier.other10.1016/S0006-3495(97)78706-X
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/162780
Abstractdc.description.abstractInositol 1,4,5-trisphosphate (InsP3) binds to its receptor channels and causes liberation of Ca2+ from intracellular stores, frequently in an oscillatory manner. In addition to InsP3, the activation and inactivation properties of these intracellular channels are controlled by Ca2+. We studied the influence of Ca2+ entry on the kinetics of InsP3-triggered oscillations in cytosolic calcium ([Ca2+](i)) in gonadotrophs stimulated with gonadotropin-releasing hormone, an agonist that activates InsP3 production. The natural expression of voltage-gated Ca2+ channels (VGCC) in these cells was employed to manipulate Ca2+ entry by voltage clamping the cells at different membrane potentials (V(m)). Under physiological conditions, the frequency of the GnRH-induced oscillations increased with time, while the amplitude decreased, until both reached stable values. However, in cells with V(m) held at -50 mV or lower, both parameters progressively decreased until the signal was abolished. These effects
Lenguagedc.language.isoen
Publisherdc.publisherBiophysical Society
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceBiophysical Journal
Keywordsdc.subjectBiophysics
Títulodc.titleModulation of the kinetics of inositol 1,4,5-trisphosphate-induced [Ca2+](i) oscillations by calcium entry in pituitary gonadotrophs
Document typedc.typeArtículo de revista
dcterms.accessRightsdcterms.accessRightsAcceso Abierto
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile