Show simple item record

Authordc.contributor.authorHidalgo Tapia, María Cecilia 
Authordc.contributor.authorDonoso Laurent, Paulina 
Authordc.contributor.authorRodriguez, Patricio H. 
Admission datedc.date.accessioned2019-01-29T16:00:07Z
Available datedc.date.available2019-01-29T16:00:07Z
Publication datedc.date.issued1996
Cita de ítemdc.identifier.citationBiophysical Journal, Volumen 71, Issue 4, 2018, Pages 2130-2137
Identifierdc.identifier.issn00063495
Identifierdc.identifier.other10.1016/S0006-3495(96)79413-4
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/163036
Abstractdc.description.abstractCalsequestrin, a high-capacity, intermediate-affinity, calcium-binding protein present in the lumen of sarcoplasmic reticulum, undergoes extensive calcium-induced conformational changes at neutral pH that cause distinct intrinsic fluorescence changes. The results reported in this work indicate that pH has a marked effect on these calcium-induced intrinsic fluorescence changes, as well as on calorimetric changes produced by the addition of Ca2+ to calsequestrin. The addition of Ca2+ at neutral pH produced a marked and cooperative increase in calsequestrin intrinsic fluorescence. In contrast, at pH 6.0 calsequestrin's intrinsic fluorescence was not affected by the addition of Ca2+, and the same intrinsic fluorescence as that measured in millimolar calcium at neutral pH was obtained. The magnitude and the cooperativity of the calcium-induced intrinsic fluorescence changes decreased as either [H+] or [K+] increased. The evolution of heat production, determined by microcalorimetry, observed
Lenguagedc.language.isoen
Publisherdc.publisherBiophysical Society
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceBiophysical Journal
Keywordsdc.subjectBiophysics
Títulodc.titleProtons induce calsequestrin conformational changes
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


Files in this item

Icon

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile