Activation of protein tyrosine kinase: A possible requirement for fixed-bacteria and lipopolysaccharide-induced increase in human natural killer cell activity
Author
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Puente, J.
Author
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Salas, M. A.
Author
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Canon, C.
Author
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Miranda, D.
Author
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Wolf, M. E.
Author
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Mosnaim, A. D.
Admission date
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2019-01-29T16:00:11Z
Available date
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2019-01-29T16:00:11Z
Publication date
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1996
Cita de ítem
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International Journal of Clinical Pharmacology and Therapeutics, Volumen 34, Issue 5, 2018, Pages 212-218
Identifier
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09461965
Identifier
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https://repositorio.uchile.cl/handle/2250/163054
Abstract
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Preincubation of peripheral blood lymphocytes (PBL) from drug-free, healthy volunteers with either the protein tyrosine kinase inhibitor genistein (GNT, n = 10, final concentration 200 μM) or the protein kinase A activator dybutiryl-cyclic-AMP (cAMP, n = 11, final concentration 10 μM), resulted in a significant inhibition of natural killer cell activity (NKCA, expressed as percentage of specific chromium release). With the exception of 4 out of the 11 cAMP-treated samples, individual values for NKCA in the drug preincubated specimens were at least 20% below the same subject baseline activity, furthermore, NCK lytic function was non-detectable in 4 out of the 10 and in 1 out of the 11 samples presented with either GNT or cAMP, respectively. PBL preincubation with glutaraldehyde-fixed Gram-negative bacteria (GNB, n = 13, final GNB-to-effector cell ratio of 50: 1) resulted in a statistically significant increase in NKCA (baseline (x ± SD) of 21.6 ± 16.4 and bacteria treated samples of 41.
International Journal of Clinical Pharmacology and Therapeutics
Keywords
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cAMP
Keywords
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Genistein
Keywords
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Glutaraldehyde-fixed Gram-negative bacteria
Keywords
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Lipopolysaccharide
Keywords
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Natural killer cell activity
Keywords
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Protein kinase A
Keywords
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Protein tyrosine kinase
Título
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Activation of protein tyrosine kinase: A possible requirement for fixed-bacteria and lipopolysaccharide-induced increase in human natural killer cell activity