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Authordc.contributor.authorSierralta, Jimena 
Authordc.contributor.authorFill, Michael 
Authordc.contributor.authorSuárez Isla, Benjamín 
Admission datedc.date.accessioned2019-01-29T16:00:20Z
Available datedc.date.available2019-01-29T16:00:20Z
Publication datedc.date.issued1996
Cita de ítemdc.identifier.citationJournal of Biological Chemistry, Volumen 271, Issue 29, 2018, Pages 17028-17034
Identifierdc.identifier.issn00219258
Identifierdc.identifier.other10.1074/jbc.271.29.17028
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/163090
Abstractdc.description.abstractThe functional heterogeneity of the ryanodine receptor (RyR) channels in avian cerebellum was defined. Heavy endoplasmic reticulum microsomes had significant levels of ryanodine and inositol 1,4,5-trisphosphate binding. Scatchard analysis and kinetic studies indicated the existence of at least two distinct ryanodine binding sites. Ryanodine binding was calcium-dependent but was not significantly enhanced by caffeine. Incorporation of microsomes into planar lipid bilayers revealed ion channels with pharmacological features (calcium, magnesium, ATP, and caffeine sensitivity) similar to the RyR channels found in mammalian striated muscle. Despite a wide range of unitary conductances (220-500 picosiemens, symmetrical cesium methanesulfonate), ryanodine locked both channels into a characteristic slow gating subconductance state, positively identifying them as RyR channels. Two populations of avian RyR channels were functionally distinguished by single channel calcium sensitivity. One popula
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceJournal of Biological Chemistry
Keywordsdc.subjectBiochemistry
Keywordsdc.subjectMolecular Biology
Keywordsdc.subjectCell Biology
Títulodc.titleFunctionally heterogenous ryanodine receptors in avian cerebellum
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile