Show simple item record

Authordc.contributor.authorCastro, Andrea 
Authordc.contributor.authorCastro, Olga 
Authordc.contributor.authorTroncoso, José Luis 
Authordc.contributor.authorKohen Skop, Paulina 
Authordc.contributor.authorSimon, Carlos 
Authordc.contributor.authorVega Blanco, María Margarita 
Authordc.contributor.authorDevoto, Luigi 
Admission datedc.date.accessioned2019-01-29T17:15:54Z
Available datedc.date.available2019-01-29T17:15:54Z
Publication datedc.date.issued1998
Cita de ítemdc.identifier.citationHuman Reproduction, Volumen 13, Issue 6, 2018, Pages 1584-1589
Identifierdc.identifier.issn02681161
Identifierdc.identifier.other10.1093/humrep/13.6.1584
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/163353
Abstractdc.description.abstractFlow cytometry analysis of luteal cells revealed that an important proportion of these cells are leukocytes. The percentage of leukocytes was higher in the early (42 ± 4) and late (35 ± 3) luteal phases than in the mid-luteal (24 ± 2) phase. However, the proportion of macrophages did not differ between the luteal stages. The flow cytometric properties correlated with cellular size and granularity were not reliable as discriminators of luteal cell subpopulations. Therefore, to assess the contribution of luteal leukocytes, these cells were completely removed from luteal cell suspensions (total cells), by a negative selection procedure (immunomagnetic separation). The functional role of leukocytes in mid-luteal steroidogenesis was assessed, in total as well as leukocyte-depleted cells. Progesterone production was found to have increased 2.2-fold in leukocyte-depleted cell cultures, in comparison with total cells under basal conditions. However, the response to human chorionic gonadotrophi
Lenguagedc.language.isoen
Publisherdc.publisherOxford University Press
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceHuman Reproduction
Keywordsdc.subjectCorpus luteum
Keywordsdc.subjectFlow cytometry
Keywordsdc.subjectInterleukin-1β
Keywordsdc.subjectLeukocyte
Keywordsdc.subjectSteroidogenesis
Títulodc.titleLuteal leukocytes are modulators of the steroidogenic process of human mid-luteal cells
Document typedc.typeArtículo de revista
dcterms.accessRightsdcterms.accessRightsAcceso Abierto
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


Files in this item

Icon

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile