Intracellular Ca2+ homeostasis in rat round spermatids
Author
dc.contributor.author
Berrios, Julio
Author
dc.contributor.author
Osses, Nelson
Author
dc.contributor.author
Opazo, Carlos
Author
dc.contributor.author
Arenas, Gloria
Author
dc.contributor.author
Mercado, Luis
Author
dc.contributor.author
Benos, Dale J.
Author
dc.contributor.author
Reyes, Juan G.
Admission date
dc.date.accessioned
2019-01-29T17:16:01Z
Available date
dc.date.available
2019-01-29T17:16:01Z
Publication date
dc.date.issued
1998
Cita de ítem
dc.identifier.citation
Biology of the Cell (1998) 90, 391-398
Identifier
dc.identifier.issn
02484900
Identifier
dc.identifier.other
10.1016/S0248-4900(98)80088-9
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/163388
Abstract
dc.description.abstract
Intracellular calcium, [Caz+], can regulate meiotic progression of mammalian oocytes. However,
the role of [Ca2+], in the regulation of the spermatogenic process and its cellular homeostatic mechanisms
in spermatogenic cells has not been elucidated. Using intracellular fluorescent probes for
Ca2f and immunodetection of plasma membrane (PM) Ca2+-ATPases, we report that: a) rat round
spermatids maintain [Ca*+]i levels of 60 k 5 nM (SEM), as estimated with flu03 in single cells or
fura- in cells in suspension; b) these cells regulate [Ca2+], by actively extruding it using a PM Ca2+-
ATPase; c) rat spermatids also actively transport Ca *+ by sarco-endoplasmic reticulum type
ATPases (SERCA); d) rat spermatids possess non-mitochondrial intracellular Ca2+, stores insensitive
to thapsigargin but releasable by ionomycin; and e) rat spermatids do not activate Ca2+ entry
mechanisms by the release of Ca *+ from SERCA-regulated stores. These results demonstrate that
rat round spermatids can generate modulated intracellular Ca2+ signals upon activation of Ca2+
channels or Ca2+ release from intracellular stores.