Show simple item record

Authordc.contributor.authorPillutla, Renuka C. 
Authordc.contributor.authorYue, Zhenyu 
Authordc.contributor.authorMaldonado, Edio 
Authordc.contributor.authorShatkin, Aaron J. 
Admission datedc.date.accessioned2019-01-29T17:16:07Z
Available datedc.date.available2019-01-29T17:16:07Z
Publication datedc.date.issued1998
Cita de ítemdc.identifier.citationJournal of Biological Chemistry, Volumen 273, Issue 34, 2018, Pages 21443-21446
Identifierdc.identifier.issn00219258
Identifierdc.identifier.other10.1074/jbc.273.34.21443
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/163413
Abstractdc.description.abstractGuanine N-7 methylation is an essential step in the formation of the m7GpppN cap structure that is characteristic of eukaryotic mRNA 5' ends. The terminal 7-methylguanosine is recognized by cap-binding proteins that facilitate key events in gene expression including mRNA processing, transport, and translation. Here we describe the cloning, primary structure, and properties of human RNA (guanine-7-)methyltransferase. Sequence alignment of the 476-amino acid human protein with the corresponding yeast ABD1 enzyme demonstrated the presence of several conserved motifs known to be required for methyltransferase activity. We also identified a Drosophila open reading frame that encodes a putative RNA (guanine-7-)methyltransferase and contains these motifs. Recombinant human methyltransferase transferred a methyl group from S-adenosylmethionine to GpppG 5'ends, which are formed on RNA polymerase II transcripts by the sequential action of RNA 5'-triphosphatase and guanylyltransferase activities
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceJournal of Biological Chemistry
Keywordsdc.subjectBiochemistry
Keywordsdc.subjectMolecular Biology
Keywordsdc.subjectCell Biology
Títulodc.titleRecombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


Files in this item

Icon

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile