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Authordc.contributor.authorMora Ramírez, Pablo Eduardo 
Authordc.contributor.authorToledo, Héctor 
Authordc.contributor.authorDominique Guiliani, Nicolas Simon 
Authordc.contributor.authorJerez, Carlos A. 
Admission datedc.date.accessioned2019-01-29T17:51:05Z
Available datedc.date.available2019-01-29T17:51:05Z
Publication datedc.date.issued2002
Cita de ítemdc.identifier.citationApplied and Environmental Microbiology, Volumen 68, Issue 4, 2018, Pages 1837-1845
Identifierdc.identifier.issn00992240
Identifierdc.identifier.other10.1128/AEM.68.4.1837-1845.2002
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/163498
Abstractdc.description.abstractBy proteomic analysis we found a 21-kDa protein (P21) from Acidithiobacillus ferrooxidans ATCC 19859 whose synthesis was greatly increased by growth of the bacteria in pyrite, thiosulfate, elemental sulfur, CuS, and ZnS and was almost completely repressed by growth in ferrous iron. After we determined the N-terminal amino acid sequence of P21, we used the available preliminary genomic sequence of A. ferrooxidans ATCC 23270 to isolate the DNA region containing the p21 gene. The nucleotide sequence of this DNA fragment contained a putative open reading frame (ORF) coding for a 23-kDa protein. This difference in size was due to the presence of a putative signal peptide in the ORF coding for P21. When p21 was cloned and overexpressed in Escherichia coli, the signal peptide was removed, resulting in a mature protein with a molecular mass of 21 kDa and a calculated isoelectric point of 9.18. P21 exhibited 27% identity and 42% similarity to the Deinococcus radiodurans thiosulfate-sulfur trans
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceApplied and Environmental Microbiology
Keywordsdc.subjectBiotechnology
Keywordsdc.subjectFood Science
Keywordsdc.subjectApplied Microbiology and Biotechnology
Keywordsdc.subjectEcology
Títulodc.titleAn exported rhodanese-like protein is induced during growth of Acidithiobacillus ferrooxidans in metal sulfides and different sulfur compounds
Document typedc.typeArtículo de revista
dcterms.accessRightsdcterms.accessRightsAcceso Abierto
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile