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Authordc.contributor.authorVillavicencio, 
Authordc.contributor.authorIñíguez Vila, Germán 
Authordc.contributor.authorJohnson, 
Authordc.contributor.authorGabler Neale, Fernando 
Authordc.contributor.authorPalomino, 
Authordc.contributor.authorVega Vega, Luis Felipe 
Admission datedc.date.accessioned2019-01-29T17:51:49Z
Available datedc.date.available2019-01-29T17:51:49Z
Publication datedc.date.issued2002
Cita de ítemdc.identifier.citationReproduction, Volumen 124, Issue 4, 2018, Pages 501-508
Identifierdc.identifier.issn14701626
Identifierdc.identifier.other10.1530/rep.0.1240501
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/163571
Abstractdc.description.abstractThe aim of the present study was to investigate the action of insulin-like growth factor I (IGF-I) and insulin-like growth factor-binding protein 3 (IGFBP-3) on steroidogenesis and apoptosis in human corpus luteum during the midluteal phase. Slices from corpora lutea were incubated for 4 h with IGF-I or IGFBP-3. Progesterone, oestradiol, androstenedione and testosterone concentrations were determined by radioimmunoassay; caspase 3 expression was assessed by immunohistochemistry; bcl-2, bax and P450arom expression were assessed by RT-PCR; and apoptosis was detected by in situ terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling. The results showed that addition of IGF-I stimulated progesterone production (150%, P < 0.01), oestradiol production (65%, P < 0.05) and bcl-2 gene expression (approximately 200%, P < 0.05), but decreased apoptosis (P < 0.05). In contrast, IGFBP-3 reduced steroid production and increased bax gene expression and the percentage of apoptotic cells
Lenguagedc.language.isoen
Publisherdc.publisherJournals of Reproduction and Fertility Ltd
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceReproduction
Keywordsdc.subjectReproductive Medicine
Keywordsdc.subjectEmbryology
Keywordsdc.subjectEndocrinology
Keywordsdc.subjectObstetrics and Gynecology
Keywordsdc.subjectCell Biology
Títulodc.titleRegulation of steroid synthesis and apoptosis by insulin-like growth factor I and insulin-like growth factor binding protein 3 in human corpus luteum during the midluteal phase
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile