Effect of ethanol on regulation of (Na + K)-adenosine triphosphatase by aldosterone and dexamethasone in cultured renal papillary collecting duct cells
Author
dc.contributor.author
Rodrigo Salinas, Ramón
Author
dc.contributor.author
Rivera, Gonzalo
Author
dc.contributor.author
Lucero, Yalola
Author
dc.contributor.author
Larraguibel, Constanza
Admission date
dc.date.accessioned
2019-01-29T17:51:52Z
Available date
dc.date.available
2019-01-29T17:51:52Z
Publication date
dc.date.issued
2002
Cita de ítem
dc.identifier.citation
Endocrine, vol. 19, no. 3, 301–304, December 2002
Identifier
dc.identifier.issn
0969711X
Identifier
dc.identifier.other
10.1385/ENDO:19:3:301
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/163592
Abstract
dc.description.abstract
Chronic ethanol exposure causes alterations in biologic
membranes of different cell types. (Na + K)–adenosine
triphosphatase (ATPase), a membrane-bound enzyme
inhibited by the acute presence of ethanol, increases
its activity in rat kidney after chronic ethanol consumption.
The aim of this investigation was to evaluate the
effect of ethanol on the modulation of (Na + K)-ATPase
by glucocorticoids and mineralocorticoids in renal
papillary collecting duct cells. Cultured renal papillary
collecting duct cells were exposed to a medium containing
150 mM ethanol plus either 100 nM aldosterone
or 10 nM dexamethasone. Control groups were
cultured in the absence of ethanol and/or the hormones.
Mg2+-ATPase was used as control enzyme. The activity
of ATPases was measured by ATP hydrolysis. Ethanol
increased the activities of (Na + K)-ATPase and Mg2+-
ATPase in 29 and 33% of controls, respectively; only
(Na + K)-ATPase activity was elevated in the presence of
aldosterone or dexamethasone, whereas Mg2+-ATPase
was unaltered by these hormones. The effects of aldosterone
and dexamethasone on (Na + K)-ATPase activity
were augmented by ethanol in 50 and 19% of controls,
respectively. These results suggest that ethanol treatment
enhances the upregulation of (Na + K)-ATPase
activity by both aldosterone and dexamethasone, in
cultured renal papillary collecting duct cells.
Effect of ethanol on regulation of (Na + K)-adenosine triphosphatase by aldosterone and dexamethasone in cultured renal papillary collecting duct cells