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Autordc.contributor.authorMarkoski, Melissa M. 
Autordc.contributor.authorBizarro, Cristiano V. 
Autordc.contributor.authorFarias, Sandra 
Autordc.contributor.authorEspinoza, Ingrid 
Autordc.contributor.authorGalanti Garrone, Norbel 
Autordc.contributor.authorZaha, Arnaldo 
Autordc.contributor.authorFerreira, Henrique B. 
Fecha ingresodc.date.accessioned2019-01-29T17:51:58Z
Fecha disponibledc.date.available2019-01-29T17:51:58Z
Fecha de publicacióndc.date.issued2003
Cita de ítemdc.identifier.citationJournal of Parasitology, Volumen 89, Issue 1, 2018, Pages 27-34
Identificadordc.identifier.issn00223395
Identificadordc.identifier.other10.1645/0022-3395(2003)089[0027:IVSIOM]2.0.CO;2
Identificadordc.identifier.urihttps://repositorio.uchile.cl/handle/2250/163623
Resumendc.description.abstractMesocestoides corti is a suitable model for studying cestode development because of its ability to reproduce asexually and segment in vitro. The cultured parasite is also capable of sexual differentiation and, probably, reproduction. To establish conditions that increase the efficiency of in vitro M. corti larvae (tetrathyridia) segmentation, we tested the effects of an inducing agent and some physical parameters in cultures. We found that a 5% CO2-95% N2 gas phase, an incubation temperature of 39 C (instead of 37 C), and a 24-hr pretreatment with trypsin (105 BAEE/ml, BAEE = Nα-benzoil-L-arginine ethyl ester unit of trypsin activity) in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 20% fetal bovine serum (FBS) are able to increase individually or synergistically the segmentation rate of tetrathyridia. A segmentation rate of up to 100% was achieved on day 4 of culture, when all these conditions were used simultaneously, in comparison with an average rate of 40% o
Idiomadc.language.isoen
Tipo de licenciadc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link a Licenciadc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Fuentedc.sourceJournal of Parasitology
Palabras clavesdc.subjectParasitology
Palabras clavesdc.subjectEcology, Evolution, Behavior and Systematics
Títulodc.titleIn vitro segmentation induction of Mesocestoides corti (cestoda) tetrathyridia
Tipo de documentodc.typeArtículo de revista
Catalogadoruchile.catalogadorSCOPUS
Indizaciónuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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