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Authordc.contributor.authorEltit Ortega, José Miguel 
Authordc.contributor.authorGarcía, Alejandra 
Authordc.contributor.authorHidalgo, Jorge 
Authordc.contributor.authorLiberona, José L. 
Authordc.contributor.authorChiong Lay, Mario 
Authordc.contributor.authorLavandero González, Sergio 
Authordc.contributor.authorMaldonado, Edio 
Authordc.contributor.authorJaimovich Pérez, Enrique 
Cita de ítemdc.identifier.citationJournal of Biological Chemistry, Volumen 281, Issue 17, 2018, Pages 12143-12154
Abstractdc.description.abstractTetanic electrical stimulation of myotubes evokes a ryanodine receptor-related fast calcium signal, during the stimulation, followed by a phospholipase C/inositol 1,4,5-trisphosphate-dependent slow calcium signal few seconds after stimulus end. L-type calcium channels (Cav 1.1, dihydropyridine receptors) acting as voltage sensors activate an unknown signaling pathway involved in phospholipase C activation. We demonstrated that both G protein and phosphatidylinositol 3-kinase were activated by electrical stimulation, and both the inositol 1,4,5-trisphosphate rise and slow calcium signal induced by electrical stimulation were blocked by pertussis toxin, by a Gβγ scavenger peptide, and by phosphatidylinositol 3-kinase inhibitors. Immunofluorescence using anti-phosphatidylinositol 3-kinase γ antibodies showed a clear location in striations within the cytoplasm, consistent with a position near the I band region of the sarcomere. The time course of phosphatidylinositol 3-kinase activation, m
Publisherdc.publisherAmerican Society for Biochemistry and Molecular Biology Inc.
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.uri
Sourcedc.sourceJournal of Biological Chemistry
Keywordsdc.subjectMolecular Biology
Keywordsdc.subjectCell Biology
Títulodc.titleMembrane electrical activity elicits inositol 1,4,5-trisphosphate-dependent slow Ca2+ signals through a Gβγ/phosphatidylinositol 3-kinase γ pathway in skeletal myotubes
Document typedc.typeArtículo de revista
Indexationuchile.indexArtículo de publicación SCOPUS

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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile