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Authordc.contributor.authorStutzin Schottlander, Andrés 
Authordc.contributor.authorEguiguren, Ana Luisa 
Authordc.contributor.authorCid, L. Pablo 
Authordc.contributor.authorSepúlveda, Francisco V. 
Cita de ítemdc.identifier.citationAmerican Journal of Physiology, Volumen 273, Issue 3 PART 1, 2018,
Abstractdc.description.abstractOrganic osmolyte and halide permeabil-ity pathways activated in epithelial HeLa cells by osmotically induced cell swelling were studied using electrophysiological and radiotracer efflux techniques. On hypotonie challenge, HeLa cells responded by activating an efflux pathway for [3H]taurine and a swelling-induced outwardly rectifying Cl- channel. Removal of extracellular Cl-, or its replacement by a less permeable anion, enhanced taurine efflux and decreased the inward current (Cl- efflux). The effect of Cl- removal on taurine efflux was not a consequence of changes in membrane potential. The degree of deactivation of the Cl- current at depolarized potentials was also Cl- dependent, suggesting that external Cl- is necessary for channel activity. The Cl- channel inhibitors 1,9-dideoxyforskolin, tamoxifen, and 4,4′diisothiocyanostilbene-2,2′-disulfonic acid (DIDS) inhibited swelling-activated taurine efflux, with DIDS being the most potent, at variance with the sensitivity of the Cl- chan
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.uri
Sourcedc.sourceAmerican Journal of Physiology
Keywordsdc.subjectChloride channel
Keywordsdc.subjectPermeability pathways
Keywordsdc.subjectRegulatory volume decrease
Keywordsdc.subjectTaurine efflux
Keywordsdc.subjectVolume-activated chloride currents
Títulodc.titleModulation by extracellular Cl- of volume-activated organic osmolyte and halide permeabilities in HeLa cells
Document typedc.typeArtículo de revista
Indexationuchile.indexArtículo de publicación SCOPUS

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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile