Liolaemus tenuis is a widely distributed arboreal lizard species in central-southern Chile. Although two subspecies, L. t. tenuis and L. t. punctatissimus, have been described based on sexual dimorphism, their characteristic have not been accurately demarcated. Therefore, both traditional and geometric morphometrics were used to study the morphological variation of L. tenuis. Four specific questions were addressed: (1) Does population morphological variation occur along a latitudinal gradient, given its wide geographic distribution in a climactic gradient? (2) Does L. tenuis present sexual dimorphism? (3) In the case of dimorphism, what is the variation along a latitudinal gradient? (4) Are these subspecies morphologically distinguishable? The study was performed in a variety of locations, including the following six bioclimatic regions of Chile: arid mediterranean, semi-arid mediterranean, sub-humid mediterranean, humid mediterranean, per-humid mediterranean, and oceanic with mediterr