Show simple item record

Authordc.contributor.authorPuente, Javier 
Authordc.contributor.authorMiranda, Dante 
Authordc.contributor.authorBlanco, Luz 
Authordc.contributor.authorWolf, Marion E. 
Authordc.contributor.authorMosnaim, Aron D. 
Admission datedc.date.accessioned2019-03-11T12:53:04Z
Available datedc.date.available2019-03-11T12:53:04Z
Publication datedc.date.issued1998
Cita de ítemdc.identifier.citationFASEB Journal, Volumen 12, Issue 5, 2018,
Identifierdc.identifier.issn08926638
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/164219
Abstractdc.description.abstractThe increase in human natural killer cell activity (NKCA) in samples of peripheral blood mononuclear cells (PBMC) elicited by their incubation with bacterial lipopolysaccharide (LPS), appears to result from an indirect process; various cytokines, expressed after an initial LPS activation of mCD14 monocyte receptors, enhance NK cell lytic function. In an attempt to correlate changes in NKCA with NKC signal transduction mechanisms mediated by LPS, we have analyzed the effect of this substance on the lytic activity and tyrosine phosphorylation pattern of PBMC samples before and after NKC purification (P). PBMC, obtained from healthy, drug-free volunteers (n=7) of the Blood Bank of the J.J. Aguirre Hospital, University of Chile were monocyte depleted (plastic adherence), washed (pH 7.4 buffered-saline) and suspended in fetal bovine serum heat-inactivated medium. Purified CD56+ cells (CD56+ > 95%, flow cytometry) were obtained by immunomagnetic isolation and NKCA was determined using K-562
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceFASEB Journal
Keywordsdc.subjectBiotechnology
Keywordsdc.subjectBiochemistry
Keywordsdc.subjectMolecular Biology
Keywordsdc.subjectGenetics
Títulodc.titleNatural killer cell signal transduction mechanism mediated by lipopolysaccharide activation
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


Files in this item

Icon

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile