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Authordc.contributor.authorJuretic Díaz, Nevenka Militza
Authordc.contributor.authorUrzúa, Ulises
Authordc.contributor.authorMunroe, David J.
Authordc.contributor.authorJaimovich Pérez, Enrique
Authordc.contributor.authorRiveros, Nora
Admission datedc.date.accessioned2019-03-11T12:53:18Z
Available datedc.date.available2019-03-11T12:53:18Z
Publication datedc.date.issued2007
Cita de ítemdc.identifier.citationJournal of Cellular Physiology, Volumen 210, Issue 3, 2018, Pages 819-830
Identifierdc.identifier.issn00219541
Identifierdc.identifier.other10.1002/jcp.20902
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/164270
Abstractdc.description.abstractSkeletal muscle is a highly plastic tissue with a remarkable capacity to adapt itself to challenges imposed by contractile activity. Adaptive response, that include hypertrophy and activation of oxidative mechanisms have been associated with transient changes in transcriptional activity of specific genes. To define the set of genes regulated by a depolarizing stimulus, we used 22 K mouse oligonucleotide microarrays. Total RNA from C2C12 myotubes was obtained at 2, 4, 18, and 24 h after high K+ stimulation. cDNA from control and depolarized samples was labeled with cyanine 3 or 5 dyes prior to microarray hybridization. Loess normalization followed by statistical analysis resulted in 423 differentially expressed genes using an unadjusted P-value ≤0.01 as cut off. Depolarization affects transcriptional activity of a limited number of genes, mainly associated with metabolism, cell communication and response to stress. A number of genes related to Ca 2+ signaling pathways are induced at 4 h
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceJournal of Cellular Physiology
Keywordsdc.subjectMedicine (all)
Keywordsdc.subjectPhysiology
Keywordsdc.subjectClinical Biochemistry
Keywordsdc.subjectCell Biology
Títulodc.titleDifferential gene expression in skeletal muscle cells after membrane depolarization
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorSCOPUS
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile