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Authordc.contributor.authorGarcía Rudaz, Cecilia 
Authordc.contributor.authorLuna, Félix 
Authordc.contributor.authorTapia, Verónica 
Authordc.contributor.authorKerr, Bredford 
Authordc.contributor.authorColgin, Lois 
Authordc.contributor.authorGalimi, Francesco 
Authordc.contributor.authorDissen, Gregory A. 
Authordc.contributor.authorRawlings, Neil D. 
Authordc.contributor.authorOjeda, Sergio R. 
Admission datedc.date.accessioned2019-03-11T12:54:02Z
Available datedc.date.available2019-03-11T12:54:02Z
Publication datedc.date.issued2007
Cita de ítemdc.identifier.citationDevelopment 134, 945-957 (2007)
Identifierdc.identifier.issn09501991
Identifierdc.identifier.other10.1242/dev.02795
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/164317
Abstractdc.description.abstractIn rodents, the formation of ovarian follicles occurs after birth. In recent years, several factors required for follicular assembly and the growth of the newly formed follicles have been identified. We now describe a novel gene, Fxna, identified by differential display in the neonatal rat ovary. Fxna encodes an mRNA of 5.4 kb, and a protein of 898 amino acids. Fxna is a transmembrane metallopeptidase from family M28, localized to the endoplasmic reticulum. In the ovary, Fxna mRNA is expressed in granulosa cells; its abundance is maximal 48 hours after birth, i.e. during the initiation of follicular assembly. Reducing Fxna mRNA levels via lentiviral-mediated delivery of short hairpin RNAs to neonatal ovaries resulted in substantial loss of primordial, primary and secondary follicles, and structural disorganization of the ovary, with many abnormal follicles containing more than one oocyte and clusters of somatic cells not associated with any oocytes. These abnormalities were not attributable to either increased apoptosis or decreased proliferation of granulosa cells. The results indicate that Fxna is required for the organization of somatic cells and oocytes into discrete follicular structures. As an endoplasmic reticulum-bound peptidase, Fxna may facilitate follicular organization by processing precursor proteins required for intraovarian cell-to-cell communication.
Lenguagedc.language.isoen
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/
Sourcedc.sourceDevelopment
Keywordsdc.subjectFollicular assembly
Keywordsdc.subjectFollicular growth
Keywordsdc.subjectOvarian development
Keywordsdc.subjectPeptidases
Keywordsdc.subjectsiRNAs
Títulodc.titleFxna, a novel gene differentially expressed in the rat ovary at the time of folliculogenesis, is required for normal ovarian histogenesis
Document typedc.typeArtículo de revista
Catalogueruchile.catalogadorlaj
Indexationuchile.indexArtículo de publicación SCOPUS
uchile.cosechauchile.cosechaSI


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile